using-chronic-social-stress-to-model-postpartum-depression-lactating

Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents

Exposure to chronic stress is a reliable predictor of depressive disorders, and social stress is a common ethologically relevant stressor in both animals ...

Tufts University Cummings School of Veterinary Medicine

Prior studies have supported a role for mesolimbic dopaminergic mechanisms in the regulation of maternal behavior. Accordingly, the ventral tegmental area (VTA) and its dopaminergic projections to the nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) have been implicated in both the onset and maintenance of normal maternal behavior. To date, studies of direct manipulation of VTA neurochemistry at the onset of maternal behavior have been limited. The current study was undertaken to directly test the hypothesis that enhancement of dopaminergic transmission in the mesolimbic dopamine system can stimulate maternal activity using a pup-induced virgin model. Nulliparous female rats were stereotaxically infused with pertussis toxin (PTX 0, 0.1, or 0.3 Î¼g/hemisphere) into the VTA to chronically stimulate the activity of dopaminergic projection neurons. After 3 days of recovery, maternal responding to donor pups was tested daily, and latency (in days) to full maternal behavior was recorded. Intra-VTA PTX treatment produced a robust dose-dependent decrease in maternal behavior latency, and a long-lasting increase in locomotor activity. These effects were associated with significantly decreased dopamine D1 receptor mRNA expression in the NAc. No effects of PTX treatment on mesolimbic dopamine utilization or mPFC receptor expression were observed. The findings indicate that chronic neural activation in the VTA accelerates the onset of maternal behavior in virgin female rats via modification of the NAc dopamine D1 receptor.

Accelerated maternal responding following intra-VTA pertussis toxin treatment.

Des &eacute;tudes ant&eacute;rieures ont soutenu un r&ocirc;le pour m&eacute;canismes dopaminergiques m&eacute;solimbiques dans la r&eacute;gulation du comportement maternel. En cons&eacute;quence, l&#39;aire tegmentale ventrale (VTA) et ses projections dopaminergiques pour le noyau accumbens (NAc) et le cortex pr&eacute;frontal m&eacute;dial (MFPC) ont &eacute;t&eacute; impliqu&eacute;es dans l&#39;apparition et le maintien de la comportement maternel normal. A ce jour, les &eacute;tudes de manipulation directe de neurochimie ADV &agrave; l&#39;apparition du comportement maternel ont &eacute;t&eacute; limit&eacute;s. La pr&eacute;sente &eacute;tude visait &agrave; v&eacute;rifier directement l&#39;hypoth&egrave;se que l&#39;am&eacute;lioration de la transmission dopaminergique dans le syst&egrave;me dopaminergique m&eacute;solimbique peut stimuler l&#39;activit&eacute; maternelle en utilisant un mod&egrave;le vierge induite par le chiot. Des rats femelles nullipares &eacute;taient stereotaxically infus&eacute;es avec la toxine coquelucheuse (PTX 0, 0,1 ou 0,3 &mu;g/h&eacute;misph&egrave;re) dans l&#39;aire tegmentale ventrale &agrave; chroniquement stimulent l&#39;activit&eacute; des neurones de projection dopaminergiques. Apr&egrave;s 3 jours de r&eacute;cup&eacute;ration, maternelle r&eacute;pondant &agrave; petits donateurs a &eacute;t&eacute; test&eacute; par jour et temps de latence (en jours) au comportement plein de maternelle a &eacute;t&eacute; enregistr&eacute;. Traitement intra-VTA PTX a provoqu&eacute; une diminution dose-d&eacute;pendante robuste latence du comportement maternel et une augmentation durable de l&#39;activit&eacute; locomotrice. Ces effets ont &eacute;t&eacute; associ&eacute;es &agrave; une diminution importante dopamine D1, expression d&#39;ARNm des r&eacute;cepteurs au CNA. Aucun effet du traitement PTX sur expression du dopamine m&eacute;solimbique MFPC ou de l&#39;utilisation de r&eacute;cepteurs ont &eacute;t&eacute; observ&eacute;s. Les r&eacute;sultats indiquent que les chronique activation neurale dans l&#39;aire tegmentale ventrale acc&eacute;l&egrave;re l&#39;apparition du comportement maternel chez les femelles vierges par une modification du r&eacute;cepteur dopamine D1 du CNA.

Acc&eacute;l&eacute;r&eacute; maternelle r&eacute;pondre suite &agrave; un traitement de toxine pertussis intra-VTA.

Studi precedenti hanno sostenuto un ruolo per mesolimbico dopaminergico meccanismi nella regolazione del comportamento materno. Di conseguenza, l&#39;area tegmentale ventrale (VTA) e sue proiezioni dopaminergico il nucleo accumbens (NAc) e la corteccia prefrontale mediale (mPFC) sono stati implicati nell&#39;insorgenza e nel mantenimento del normale comportamento materno. Ad oggi, gli studi di manipolazione diretta di neurochimica VTA all&#39;esordio del comportamento materno sono stati limitati. L&#39;attuale studio &egrave; stato intrapreso per testare direttamente l&#39;ipotesi che potenziamento della trasmissione dopaminergica nel sistema mesolimbico dopamina pu&ograve; stimolare attivit&agrave; materna utilizzando un modello di Vergine pup-indotta. Nullipare ratti femminili erano stereotaxically infusi con tossina pertosse (PTX 0, 0,1 o 0,3 &mu;g/emisfero) in VTA cronicamente stimolare l&#39;attivit&agrave; dei neuroni dopaminergici proiezione. Dopo 3 giorni di recupero, materna risponde a cuccioli di donatore &egrave; stata testata quotidianamente e latenza (in giorni) per comportamento pieno materna &egrave; stato registrato. Trattamento intra-VTA PTX prodotta una robusta dose-dipendente diminuzione della latenza comportamento materno e un aumento di lunga durata nell&#39;attivit&agrave; dell&#39;apparato locomotore. Questi effetti sono stati associati con la dopamina significativamente diminuita espressione del mRNA del recettore D1 nel NAc. Sono stati osservati effetti di trattamento PTX sull&#39;espressione del recettore del utilizzazione o mPFC mesolimbico dopamina. I risultati indicano che cronica attivazione neurale nel VTA accelera l&#39;insorgenza del comportamento materno nei ratti femmina vergini tramite modificazione dei recettori D1 della dopamina NAc.

Accelerazione materna risponde a seguito di trattamento con tossina pertosse intra-VTA.

The use and misuse of prescription opiates in adolescent populations, and in particular, adolescent female populations, has increased dramatically in the past two decades. Given the significant role that opioids play in neuroendocrine function, exposure to opiates during this critical developmental period could have significant consequences for the female, as well as her offspring.

Multigenerational effects of adolescent morphine exposure on dopamine D2 receptor function.

L&#39;utilisation et l&#39;abus d&#39;opiac&eacute;s de prescription dans des populations adolescentes et en particuliers, adolescentes populations f&eacute;minines, a augment&eacute; consid&eacute;rablement au cours des deux derni&egrave;res d&eacute;cennies. Compte tenu du r&ocirc;le important que jouent les opio&iuml;des en fonction neuroendocrine, exposition aux opiac&eacute;s au cours de cette p&eacute;riode critique du d&eacute;veloppement pourrait avoir des cons&eacute;quences importantes pour la femelle, ainsi que sa prog&eacute;niture.

Multig&eacute;n&eacute;rationnelles effets de morphine adolescent sur dopamine D2 fonction du r&eacute;cepteur.

L&#39;uso e l&#39;abuso di oppiacei prescrizione nelle popolazioni degli adolescenti e in particolare, adolescente popolazioni femminile, &egrave; aumentato drammaticamente negli ultimi due decenni. Dato il ruolo significativo che gli oppioidi giocano in funzione neuroendocrina, esposizione di oppiacei durante questo periodo critico dello sviluppo potrebbe avere conseguenze significative per la femmina, come pure la sua prole.

Lindsay M. Carini

Biomedical Sciences

Tufts University Cummings School of Veterinary Medicine

Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents

Lindsay M. Carini

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Biomedical Sciences

Tufts University Cummings School of Veterinary Medicine

Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents

Biomedical Sciences