Syngenta Crop Protection, LLC

1 ARTICLES PUBLISHED IN JoVE

Biology

Enabling High-throughput Transgene Expression Studies Using Automated Liquid Handling for Etiolated Maize Leaf Protoplasts

Stephen B. Rigoulot¹, Jeongmoo Park¹, Jonathan Fabish¹, Erin M. Seaberry¹, Allison Parrish¹, Kerry A. Meier¹, Rachel Whinna¹, Shujie Dong¹

¹Syngenta Crop Protection, LLC

This protocol describes the creation of a randomized transfection layout using an automated liquid handler, a protoplast isolation protocol for etiolated maize leaf, and a 96-well transfection procedure using a liquid handler.