Enabling High-throughput Transgene Expression Studies Using Automated Liquid Handling for Etiolated Maize Leaf ProtoplastsStephen B. Rigoulot 1, Jeongmoo Park 1, Jonathan Fabish 1, Erin M. Seaberry 1, Allison Parrish 1, Kerry A. Meier 1, Rachel Whinna 1, Shujie Dong 1
1Syngenta Crop Protection, LLC
This protocol describes the creation of a randomized transfection layout using an automated liquid handler, a protoplast isolation protocol for etiolated maize leaf, and a 96-well transfection procedure using a liquid handler.
