Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, ³Neuroscience Program, NYU School of Medicine

Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.