Visualize Drosophila Leg Motor Neuron Axons Through the Adult CuticleWenyue Guan 1, Lalanti Venkatasubramanian 2, Myungin Baek 3, Richard S. Mann 2, Jonathan Enriquez 1
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, 3Neuroscience Program, NYU School of Medicine
Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.
