<h2 class="jove_title"><a href="https://www.jove.com/video/52486">Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles</a></h2>Bryan Wei1, Michelle K. Vhudzijena2, Joanna Robaszewski2, Peng Yin2,31Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, 2Wyss Institute for Biologically Inspired Engineering, Harvard University, 3Department of Systems Biology, Harvard Medical SchoolDNA tiling is an effective approach to make programmable nanostructures. We describe the protocols to construct complex two-dimensional shapes by the self-assembly of single-stranded DNA tiles.<h2 class="jove_title"><a href="https://www.jove.com/video/52798">Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device (ALDM) Test Systems</a></h2>Jo&#235;l Fagot, Yousri Marzouki, Pascal Huguet, Julie Gullstrand, Nicolas Claidi&#232;reCNRS, Federation 3C, and Laboratory of Cognitive Psychology, Aix-Marseille UniversityFagot &#38; Paleressompoulle1 have published an automated learning device (ALDM) aimed at testing individual cognitive abilities in semi-free ranging monkeys. The main goal of our protocol is to use a network of ALDM test units to study social cognition in non-human primates.<h2 class="jove_title"><a href="https://www.jove.com/video/52699">Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction</a></h2>Himakarnika Alluri1,2, Chinchusha Anasooya Shaji2, Matthew L. Davis1,2, Binu Tharakan1,21Department of Surgery, Texas A&amp;M University Health Science Center College of Medicine, 2Department of Surgery, Baylor Scott &amp; White HealthIschemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells. <h2 class="jove_title"><a href="https://www.jove.com/video/52703">The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants</a></h2>Michael Wisniewski1, Gilbert Neuner2, Lawrence V. Gusta31U.S Department of Agriculture, Agricultural Research Service &#40;USDA-ARS&#41;, 2Institute of Botany, University of Innsbruck, 3Department of Plant Science, University of SaskatechewanHere we present a protocol that allows one to visualize sites of ice formation and avenues of ice propagation in plants utilizing high resolution infrared thermography (HRIT).

this month in jove - assessing freezing tolerance in plants, patterning 2d shapes with dna, studying ischemia in vitro, studying social cognition in monkeys

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/99">May 2015 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, it's the middle of spring in the earth's Northern Hemisphere, so seeds are sprouting and buds are blooming. But for actively growing plants, especially those with no freezing tolerance, a cold snap can be deadly. A hard frost can dramatically alter natural ecosystems and devastate agriculture. Even more concerning, erratic weather patterns related to climate change can cause cold-susceptible plants to bud and bloom too early, then fall victim to a subsequent freeze. To protect plants from freezing, we need a better understanding of the freezing process. <a href="http://www.jove.com/video/52703/">Wisniewski et al.</a> describe a protocol to visualize this process using high-resolution infrared thermography (HRIT). Using this technology, scientists can determine how ice forms and propagates, and test various compounds that might alter the freezing process or increase freezing tolerance.
In <a href="http://www.jove.com/chem">JoVE Chemistry</a>, the principles of self-assembly have been used to engineer a variety of complex structures from biological and non-biological building blocks. This month, <a href="http://www.jove.com/video/52486/">Wei et al.</a> present detailed protocols for the self-assembly of single-stranded DNA tiles into complex 2D shapes with the tiles acting as molecular pixels on molecular canvases. These are visualized by atomic force microscopy. The modular nature of this approach allows it to be scalable, and the tiles can self-assemble into tubes and rectangles of varying sizes. This technique is also highly versatile-enabling the construction virtually any 2D shape.
In <a href="http://www.jove.com/ctm">JoVE Clinical &amp; Translational Medicine</a>, ischemic stroke happens when a blockage in an artery restricts blood flow to the brain, depriving it of oxygen and nutrients. When the blood returns, reperfusion can cause further damage by disrupting the blood-brain barrier and triggering inflammation and oxidative stress. There are many in vivo models of ischemia-reperfusion injury, and while all disease models have drawbacks, in vivo models tend to be technically challenging, with limited applicability for mechanistic studies. To help overcome some of these limitations, <a href="http://www.jove.com/video/52699">Alluri et al.</a> present an in vitro model of ischemia-reperfusion, which can be used to study the molecular mechanisms of blood-brain barrier dysfunction and signs of oxidative stress.
In <a href="http://www.jove.com/behavior">JoVE Behavior</a>, <a href="http://www.jove.com/video/52798">Fagot et al.</a> study social cognition in monkeys. In this protocol, monkeys living in social groups have free access to multiple test units. When a monkey enters a testing chamber, and reaches through the hand port, a computer recognizes the monkey through its radio frequency ID (RFID) chip and starts the experiment A touch screen displays the stimulus and a food dispenser delivers the reward. Over several years, this procedure has been shown enhance motivation in the subjects and favor complex learning-particularly the social aspects.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/99">May 2015 issue</a> of JoVE. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys a

This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys

This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys

Here's a look at what's coming up in the May 2015 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Environment, it's the middle of spring in ...

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Video: This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys

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