analysis-global-rna-synthesis-at-single-cell-level-following

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Hypoxia or lowering of the oxygen availability is involved in many physiological and pathological processes. At the molecular level, cells initiate a ...

University of Dundee, UK

We present a rare case of acute urinary retention complicated by constipation secondary to a unilateral herpes zoster reactivation in the S2-4 dermatomes of an immunocompetent female. Diagnosis was confirmed by clinical examination, negative cystoscopy and positive viral polymerase chain reaction (PCR) for herpes zoster virus. The patient was commenced on a course of oral acyclovir, the bowel symptoms resolved, and the patient was discharged with a urinary catheter in situ for an outpatient trial without catheter for 2 weeks to be followed by a course of intermittent self catheterisation pending resolution of symptoms.

Herpes zoster induced acute urinary retention in the immunocompetent female.

Cell cycle progression is an energy demanding process and requires fine-tuned metabolic regulation. Cells must overcome an energy restriction checkpoint before becoming committed to progress through the cell cycle. Aerobic organisms need oxygen for the metabolic conversion of nutrients into energy. As such, environmental oxygen is a critical signalling molecule regulating cell fate. The Hypoxia Inducible Factors (HIFs) are a family of transcription factors that respond to changes in environmental oxygen and cell energy and coordinate a transcriptional program which forms an important part of the cellular response to a hostile environment. A significant proportion of HIF-dependent transcriptional target genes, code for proteins that are involved in energy homeostasis. In this review we discuss the role of the HIF system in the regulation of energy homeostasis in response to changes in environmental oxygen and the impact on cell cycle control, and address the implications of the deregulation of this effect in cancer.

Grow₂: The HIF system, energy homeostasis and the cell cycle.

An interrupted stitch type with favorable tissue characteristics will reduce local wound complications. We describe a novel high-strength, low-tension repair for the interrupted closure of skin, cartilage, and muscle, the double loop mattress stitch, and compare it experimentally with other interrupted closure methods. The performance of the double loop mattress technique in porcine cartilage and skeletal muscle is compared with the simple, mattress, and loop mattress interrupted sutures in both a novel porcine loading chamber and mechanical model. Wound apposition is assessed by electron microscopy. The performance of the double loop mattress in vivo was confirmed using a series of 805 pediatric laparotomies/laparoscopies. The double loop mattress suture is 3.5 times stronger than the loop mattress in muscle and 1.6 times stronger in cartilage (p ≤ 0.001). Additionally, the double loop mattress reduces tissue tension by 66% compared with just 53% for the loop mattress (p ≤ 0.001). Wound gapping is equal, and wound eversion appears significantly improved (p ≤ 0.001) compared with the loop mattress in vitro. In vivo, the double loop mattress performs as well as the loop mattress and significantly better than the mattress stitch in assessments of wound eversion and dehiscence. There were no episodes of stitch extrusion in our series of patients. The mechanical advantage of its intrinsic pulley arrangement gives the double loop mattress its favorable properties. Wound dehiscence is reduced because this stitch type is stronger and exerts less tension on the tissue than the mattress stitch. We advocate the use of this novel stitch wherever a high-strength, low-tension repair is required. These properties will enhance wound repair, and its application will be useful to surgeons of all disciplines.

John Biddlestone

Centre for Gene Regulation and Expression,
College of Life Sciences,
Centre for Gene Regulation and Expression, College of Life Sciences

University of Dundee, UK

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

John Biddlestone

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University of Dundee, UK

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Centre for Gene Regulation and Expression,
College of Life Sciences,
Centre for Gene Regulation and Expression, College of Life Sciences