the-infiltration-centrifugation-technique-for-extraction-apoplastic

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using <em>Phaseolus vulgaris</em> as an Example

The apoplast is a distinct extracellular compartment in plant tissues that lies outside the plasma membrane and includes the cell wall. The apoplastic ...

University of Oxford

The bacterial plant pathogen Pseudomonas syringae causes economically important diseases of a wide variety of plant species and is used as a model organism to understand the molecular basis of plant disease. Much existing research into P. syringae-plant interactions has focused on the molecular basis of plant disease resistance and the role of secreted effector proteins in the suppression of plant defences. However, researchers have speculated that the diverse array of effectors, toxins and hormones produced by this pathogen also play an important role in manipulating plant metabolism to promote infection. Recent advances in metabolomics, genomics, transcriptomics and metabolic modelling offer new opportunities to address this question and generate a system-level understanding of metabolic interactions at the host-pathogen interface.

The metabolic interface between Pseudomonas syringae and plant cells.

The past two decades have seen an increasing number of virulent infectious diseases in natural populations and managed landscapes. In both animals and plants, an unprecedented number of fungal and fungal-like diseases have recently caused some of the most severe die-offs and extinctions ever witnessed in wild species, and are jeopardizing food security. Human activity is intensifying fungal disease dispersal by modifying natural environments and thus creating new opportunities for evolution. We argue that nascent fungal infections will cause increasing attrition of biodiversity, with wider implications for human and ecosystem health, unless steps are taken to tighten biosecurity worldwide.

Sarah McCraw

Department of Plant Sciences

University of Oxford

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Sarah McCraw

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Plant Sciences

University of Oxford

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Department of Plant Sciences