remote-limb-ischemic-preconditioning-neuroprotective-technique

Remote Limb Ischemic Preconditioning:  A Neuroprotective Technique in Rodents

Sublethal ischemia protects tissues against subsequent, more severe ischemia through the upregulation of endogenous mechanisms in the affected tissue. ...

using-electroretinogram-to-assess-function-rodent-retina-protective

Using the Electroretinogram to Assess Function in the Rodent Retina and the Protective Effects of Remote Limb Ischemic Preconditioning

The ERG is the sum of all retinal activity. The ERG is usually recorded from the cornea, which acts as an antenna that collects and sums signals from the ...

University of Sydney

Synchrotron Fourier transform-infrared (FT-IR) and Raman microspectroscopy were applied to investigate changes in the molecular architecture of mouse oocytes and demonstrate the overall morphology of the maturing oocyte. Here we show that differences were identified between immature mouse oocytes at the germinal vesicle (GV) and mature metaphase II (MII) stage when using this technology, without the introduction of any extrinsic markers, labels, or dyes. GV mouse oocytes were found to have a small, centrally located lipid deposit and another larger polar deposit of similar composition. MII oocytes have very large, centrally located lipid deposits. Each lipid deposit for both cell types contains an inner and outer lipid environment that differs in composition. To assess interoocyte variability, line scans were recorded across the diameter of the oocytes and compared from three independent trials (GV, n = 91; MII, n = 172), and the data were analyzed with principal component analysis (PCA). The average spectra and PCA loading plots show distinct and reproducible changes in the CH stretching region that can be used as molecular maturation markers. The method paves the way for developing an independent assay to assess oocyte status during maturation providing new insights into lipid distribution at the single cell level.

Shedding new light on the molecular architecture of oocytes using a combination of synchrotron Fourier transform-infrared and Raman spectroscopic mapping.

Remote ischemic preconditioning (RIP) has been found to be protective of heart and brain against ischemic injury. We have tested the effects of RIP on retinal function using the electroretinogram.

Alice Brandli

Discipline of Physiology and Bosch Institute,
Sydney Medical School,
Discipline of Physiology and Bosch Institute, Sydney Medical School

University of Sydney

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Using the Electroretinogram to Assess Function in the Rodent Retina and the Protective Effects of Remote Limb Ischemic Preconditioning

Alice Brandli

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Discipline of Physiology and Bosch Institute,Sydney Medical School,Discipline of Physiology and Bosch Institute, Sydney Medical School

University of Sydney

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Using the Electroretinogram to Assess Function in the Rodent Retina and the Protective Effects of Remote Limb Ischemic Preconditioning

Discipline of Physiology and Bosch Institute,
Sydney Medical School,
Discipline of Physiology and Bosch Institute, Sydney Medical School