probe-based-real-time-pcr-approaches-for-quantitative-measurement

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it is one of the most sensitive detection methods that ...

The University of Sydney

Death of pancreatic islet beta cells is a common feature of type 1 and 2 diabetes and often follows islet cell transplantation. Measurement of blood glucose is currently the only blunt instrument available to diagnose diabetes mellitus, and we lack tools to quantify islet cell loss or protection thereof. A class of RNA molecules (called microRNAs/miRNAs/miRs) that regulate endogenous gene expression via mRNA cleavage or translational arrest have been identified to be critical for birth, maintenance and regeneration of pancreatic beta cells. Recent demonstration that microRNAs can potentially be utilised as biomarkers due to their serum stability, has triggered increasing interest in understanding their role as regulators or biomarkers of disease. This review aims to delve into the potential of miRNA biomarkers, and whether miRNA profiles are indicators or effector of disease pathology. Furthermore, an outline for identifying and confirming islet-specific miRNA biomarkers is discussed.

Ryan Farr

Diabetes and Islet Biology Group,
NHMRC Clinical Trials Centre,
Faculty of Medicine,
Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine

The University of Sydney

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Ryan Farr

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The University of Sydney

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Diabetes and Islet Biology Group,
NHMRC Clinical Trials Centre,
Faculty of Medicine,
Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine