synthesis-structure-determination-conotoxin-piiia-isomers-with

Synthesis and Structure Determination of &#181;-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Peptides with a high number of cysteines are usually influenced regarding the three-dimensional structure by their disulfide connectivity. It is thus ...

University of Bonn

In human neonatal high pressure hydrocephalus (HPHC), diffuse white matter injury and gliosis predispose to poor neuro-developmental outcome. The underlying mechanism for diffuse white matter damage in neonatal HPHC is still unclear. Analogous to inflammatory white matter damage after neonatal hypoxemia/ischemia, we hypothesized that pro-inflammatory cytokines could be involved in neonatal HPHC. If so, early anti-inflammatory therapy could ameliorate white matter damage in HPHC, before irreversible apoptosis has occurred. In HPHC and control neonates, we therefore aimed to compare cerebrospinal fluid (CSF) concentrations of IL18, IFNgamma and sFasL (interleukin 18, interferon gamma and apoptosis marker soluble-Fas ligand, respectively).

Neonatal high pressure hydrocephalus is associated with elevation of pro-inflammatory cytokines IL-18 and IFNgamma in cerebrospinal fluid.

The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mechanisms of the bacterial terpene cyclases.

Thomas Schmitz

Pharmaceutical Biochemistry and Bioanalytics,
Pharmaceutical Institute,
Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute

University of Bonn

Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Thomas Schmitz

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Pharmaceutical Biochemistry and Bioanalytics,Pharmaceutical Institute,Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute

University of Bonn

Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Pharmaceutical Biochemistry and Bioanalytics,
Pharmaceutical Institute,
Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute