longitudinal-morphological-physiological-monitoring-three-dimensional

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Tumor spheroids have been developed as a three-dimensional (3D) cell culture model in cancer research and anti-cancer drug discovery. However, currently, ...

Lehigh University

A fast, easy, and low-cost way to fabricate transparent superhydrophobic (SHP) surfaces is developed. By simply mixing silica nanoparticles (SiNPs), polydimethylsiloxane (PDMS) and heptane to form a suspension, dip- or drop-coating the suspension onto different surfaces, transparent SHP surfaces can be obtained. By tuning the ratio of the three components above, transparency of the coating can reach more than 90% transmittance in the visible region, while static water contact angle of the coating can reach as high as 162°. Dynamic contact angle study shows the advancing contact angle and receding contact angle of water can be as high as 168° and 161°, and the resulting contact angle hysteresis can be as low as 7°. The reported facile way of fabricating transparent superhydrophobic (SHP) surfaces is potential for applications which need both optical transparency and self-cleaning capability, such as solar cells, optical equipment, and visible microfluidic chips.

A Facile Way to Fabricate Transparent Superhydrophobic Surfaces.

A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are formed and then detached by dispase while maintaining their sheet shape. These detached cell sheets are transferred to dispase-doped media under orbital shaking conditions. Assisted by the shear flow under shaking and inhibition of cell-to-extracellular matrix junctions by dispase, the cell sheets curl up and eventually tumor spheroids are formed. The average size of the spheroids can be controlled by tuning the cell sheet culturing period and spheroid shaking period. The uniformity can be controlled by a set of sieves which were home-made using stainless steel meshes. Since this method is based on simple petri-dish cell culturing and shaking, it is rather facile for forming tumor spheroids with no theoretical quantity limit. This method has been used to form HeLa, A431 and U87 MG tumor spheroids and application of the formed tumor spheroids in drug screening is also demonstrated. The viability, 3D structure, and necrosis of the spheroids are characterized.

Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity.

This paper accounts for utilization of shear induced alignment method during ceramic stereolithography. Lateral oscillation mechanism, combined with 3d printed wall pattern, was employed to generate necessary shear to align fiber in desired direction. First, semicircular channel pattern was printed to assess the effect of difference between wall direction and oscillation direction on the fiber alignment. Then, flexural strength of ceramic matrix was tested with nickel coated carbon fiber and ceramic fiber reinforcements. The results demonstrated that the shear induced alignment further improves the flexural strength compare to randomly distributed samples. Flexural strength of aligned samples with 1.0 wt% carbon fiber loading was improved by ~90% compared to randomly orientated samples and by ~333% compared to unreinforced samples. Finally, fracture surface morphology of the flexural strength test specimens was evaluated. The main fracture mechanism was observed as fiber pull-out.

Wentao Shi

Department of Bioengineering

Lehigh University

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Wentao Shi

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Bioengineering

Lehigh University

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Department of Bioengineering