pattern-triggered-oxidative-burst-seedling-growth-inhibition-assays

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in <em>Arabidopsis thaliana</em>

Plants have evolved a robust immune system to perceive pathogens and protect against disease. This paper describes two assays that can be used to measure ...

Queen's University

Plant cells detect the presence of potentially pathogenic microorganisms in the apoplast via plasma membrane-localized receptors. Activated receptors trigger phosphorylation-mediated signaling cascades that protect the cell from infection. It is thought that signaling triggered by the detection of exogenous signals, such as bacterial flagellin, can be amplified by endogenous signals, such as hormones or debris caused by cell damage, to potentiate robust immune responses. For example, perception of flagellin and other microbial molecules results in increased expression of endogenous PROPEP transcripts that give rise to AtPep peptides which also activate immune signaling. Phytohormones such as methyl-jasmonate also induce PROPEP expression, suggestive of additional hormone-mediated feedback loops that similarly amplify immune signaling. The current study aimed to determine if perception of jasmonate is genetically required for AtPep1-induced immune responses in Arabidopsis thaliana.

The jasmonate receptor COI1 is required for AtPep1-induced immune responses in Arabidopsis thaliana.

Activation of Ca signaling is a universal response to stress that allows cells to quickly respond to environmental cues. Fluctuations in cytosolic Ca are decoded in plants by Ca-sensing proteins such as Ca-dependent protein kinases (CDPKs). The perception of microbes results in an influx of Ca that activates numerous CDPKs responsible for propagating immune signals required for resistance against disease-causing pathogens. This review describes our current understanding of CDPK activation and regulation, and provides a comprehensive overview of CDPK-mediated immune signaling through interaction with various substrates.

Regulation of Plant Immune Signaling by Calcium-Dependent Protein Kinases.

Small peptides regulate the cellular coordination of growth, development, and stress tolerance in plants. In addition to direct antimicrobial activities, small secreted peptides have emerged as key signaling molecules in the plant immune response. Here, we highlight recent discoveries of several small peptides that amplify and fine-tune immune signaling.

Jacqueline Monaghan

Department of Biology

Queen's University

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Jacqueline Monaghan

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Biology

Queen's University

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Department of Biology