Sebastian J. Maerkl
Institute of Bioengineering
School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
Prof. Maerkl received B.S. degrees in Biology and in Chemistry from Fairleigh-Dickinson University in 2001. He then joined the Biophysics and Biochemistry Department at the California Institute of Technology and contributed to the early development of microfluidic technology in the laboratory of Prof. Stephen Quake. For his graduate work Prof. Maerkl was awarded the Demetriades-Tasfka-Kokalis prize for the best Caltech PhD thesis in the field of Biotechnology. He was also awarded 1st place at the Innovator’s Challenge, a competition amongst inventors and entrepreneurs from Stanford University, UC Berkeley, and Caltech. After graduating in 2008 Prof. Maerkl accepted a position as an Assistant Professor at the Ecole Polytechnique Federale de Lausanne (EPFL) in the Institute of Bioengineering and the School of Engineering and was promoted to Associate Professor with tenure in 2015. In 2012 and received the Prix SSV – Ambition for dedication to teaching and promotion of EPFL students and the school at large. Prof. Maerkl was awarded an ERC Consolidator Grant, an HFSP Program Grant, and a SystemsX.ch RTD grant. Prof. Maerkl published over 40 peer-reviewed publications and 6 patents, gave over 90 invited seminars, organised several international conferences and workshops, and serves as a reviewer for national and international funding agencies and journals. The lab hosted one Fulbright Scholar, two Whitaker and one Think Swiss Fellows. His lab is currently working at the interface of micro-engineering, systems biology, synthetic biology, and molecular diagnostics.
Integration column: Microfluidic high-throughput screening.
Integrative biology : quantitative biosciences from nano to macro Jan, 2009 | Pubmed ID: 20023788
Next generation microfluidic platforms for high-throughput protein biochemistry.
Current opinion in biotechnology Feb, 2011 | Pubmed ID: 20832278
Experimental strategies for studying transcription factor-DNA binding specificities.
Briefings in functional genomics Dec, 2010 | Pubmed ID: 20864494
A software-programmable microfluidic device for automated biology.
Lab on a chip May, 2011 | Pubmed ID: 21416077
Does positive selection drive transcription factor binding site turnover? A test with Drosophila cis-regulatory modules.
PLoS genetics Apr, 2011 | Pubmed ID: 21572512
MITOMI: a microfluidic platform for in vitro characterization of transcription factor-DNA interaction.
Methods in molecular biology (Clifton, N.J.) , 2012 | Pubmed ID: 21938622
Probing the informational and regulatory plasticity of a transcription factor DNA-binding domain.
PLoS genetics , 2012 | Pubmed ID: 22496663
Massively parallel measurements of molecular interaction kinetics on a microfluidic platform.
Proceedings of the National Academy of Sciences of the United States of America Oct, 2012 | Pubmed ID: 23012409
Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane.
Chemical communications (Cambridge, England) Feb, 2013 | Pubmed ID: 23183795
iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks.
Nucleic acids research Feb, 2013 | Pubmed ID: 23258699
A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening.
Integrative biology : quantitative biosciences from nano to macro Apr, 2013 | Pubmed ID: 23443913
Live mammalian cell arrays.
Nature methods Jun, 2013 | Pubmed ID: 23644546
Rapid synthesis of defined eukaryotic promoter libraries.
ACS synthetic biology Oct, 2012 | Pubmed ID: 23656186
Mapping the fine structure of a eukaryotic promoter input-output function.
Nature genetics Oct, 2013 | Pubmed ID: 23955598
A chemostat array enables the spatio-temporal analysis of the yeast proteome.
Proceedings of the National Academy of Sciences of the United States of America Sep, 2013 | Pubmed ID: 24019481
Implementation of cell-free biological networks at steady state.
Proceedings of the National Academy of Sciences of the United States of America Oct, 2013 | Pubmed ID: 24043836
A 1024-sample serum analyzer chip for cancer diagnostics.
Lab on a chip Aug, 2014 | Pubmed ID: 24345965
Long-term single cell analysis of S. pombe on a microfluidic microchemostat array.
PloS one , 2014 | Pubmed ID: 24710337
Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription.
Genes & development Aug, 2014 | Pubmed ID: 25085421
A microfluidic platform for high-throughput multiplexed protein quantitation.
PloS one , 2015 | Pubmed ID: 25680117
Mechanically Induced Trapping of Molecular Interactions and Its Applications.
Journal of laboratory automation Jun, 2016 | Pubmed ID: 25805850
Rapid cell-free forward engineering of novel genetic ring oscillators.
eLife Oct, 2015 | Pubmed ID: 26430766
Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering.
Nucleic acids research Apr, 2016 | Pubmed ID: 26704969
A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples.
ACS nano Jan, 2016 | Pubmed ID: 26741022
GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription-Translation System.
ACS synthetic biology 09, 2016 | Pubmed ID: 27186988
A Microfluidic Biodisplay.
ACS synthetic biology 11, 2017 | Pubmed ID: 28771313
Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles.
Analytical chemistry 01, 2018 | Pubmed ID: 29183126
Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions.
Scientific reports 05, 2018 | Pubmed ID: 29802303
Microfluidic Transfection for High-Throughput Mammalian Protein Expression.
Methods in molecular biology (Clifton, N.J.) , 2018 | Pubmed ID: 30242688
A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System.
ACS synthetic biology 02, 2019 | Pubmed ID: 30632751
Cell-free gene-regulatory network engineering with synthetic transcription factors.
Proceedings of the National Academy of Sciences of the United States of America 03, 2019 | Pubmed ID: 30850530
Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals.
Nature chemical biology 05, 2019 | Pubmed ID: 30911179
Microfluidic systems for cancer diagnostics.
Current opinion in biotechnology 10, 2020 | Pubmed ID: 31891869
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.
Frontiers in bioengineering and biotechnology , 2020 | Pubmed ID: 32266240
A partially self-regenerating synthetic cell.
Nature communications 12, 2020 | Pubmed ID: 33311509
Steady-State Cell-Free Gene Expression with Microfluidic Chemostats.
Methods in molecular biology (Clifton, N.J.) , 2021 | Pubmed ID: 33405223
A high-throughput microfluidic nanoimmunoassay for detecting anti-SARS-CoV-2 antibodies in serum or ultralow-volume blood samples.
Proceedings of the National Academy of Sciences of the United States of America 05, 2021 | Pubmed ID: 33945500
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Ardjan J. van der Linden1,
Maaruthy Yelleswarapu2,
Pascal A. Pieters1,
Zoe Swank3,
Wilhelm T. S. Huck2,
Sebastian J. Maerkl3,
Tom F. A. de Greef1,2
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology,
2Institute for Molecules and Materials, Radboud University,
3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
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