reconstitution-septin-assembly-at-membranes-to-study-biophysical

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Most cells can sense and change their shape to carry out fundamental cell processes. In many eukaryotes, the septin cytoskeleton is an integral component ...

University of North Carolina at Chapel Hill

Fungi have been found in every marine habitat that has been explored; however, the diversity and functions of fungi in the ocean are poorly understood. In this study, fungi were cultured from the marine environment in the vicinity of Woods Hole, MA, USA, including from plankton, sponge, and coral. Our sampling resulted in 35 unique species across 20 genera. We observed many isolates by time-lapse, differential interference contrast (DIC) microscopy and analyzed modes of growth and division. Several black yeasts displayed highly unconventional cell division cycles compared to those of traditional model yeast systems. Black yeasts have been found in habitats inhospitable to other life and are known for halotolerance, virulence, and stress resistance. We find that this group of yeasts also shows remarkable plasticity in terms of cell size control, modes of cell division, and cell polarity. Unexpected behaviors include division through a combination of fission and budding, production of multiple simultaneous buds, and cell division by sequential orthogonal septations. These marine-derived yeasts reveal alternative mechanisms for cell division cycles that seem likely to expand the repertoire of rules established from classic model system yeasts.

Ellysa J. D. Vogt

Curriculum in Genetics and Molecular Biology

University of North Carolina at Chapel Hill

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Ellysa J. D. Vogt

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Curriculum in Genetics and Molecular Biology

University of North Carolina at Chapel Hill

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Curriculum in Genetics and Molecular Biology