designing-bioreactor-to-improve-data-acquisition-model-throughput

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Heart failure remains the leading cause of death worldwide, creating a pressing need for better preclinical models of the human heart. Tissue engineering ...

Icahn School of Medicine at Mount Sinai

Increased signal-to-noise ratio and blood oxygenation level-dependent sensitivity at 7 Tesla (T) have the potential to enable high-resolution imaging of the human cervical spinal cord and brainstem. We propose a new two-panel radiofrequency coil design for these regions to fully exploit the advantages of ultra-high field.

7 Tesla 22-channel wrap-around coil array for cervical spinal cord and brainstem imaging.

Multi-material 3D printing with several mechanically distinct materials at once has expanded the potential applications for additive manufacturing technology. Fewer material options exist, however, for additive systems that employ vat photopolymerization (such as stereolithography, SLA, and digital light projection, DLP, 3D printers), which are more commonly used for advanced engineering prototypes and manufacturing. Those material selections that do exist are limited in their capacity for fusion due to disparate chemical and physical properties, limiting the potential mechanical range for multi-material printed composites. Here, we present an ethylene glycol phenyl ether acrylate (EGPEA)-based formulation for a polymer resin yielding a range of elastic moduli between 0.6 MPa and 33 MPa simply by altering the ratio of monomer and crosslinker feedstocks in the formulation. This simple chemistry is also well suited to form seamless adhesions between mechanically dissimilar formulations, making it a promising candidate for multi-material DLP 3D printing. Preliminary tests with these polymer formulations indicate that variability due to molecular differences between hard and soft formulations is near net shape and less than 3% of the prescribed dimensions, comparable to existing commercial DLP and SLA resins, with unique advantages of a wide range of elastomer stiffness and seamless fusion for 3D printing of structurally detailed and mechanically heterogeneous composites.

3D Printing a Mechanically-Tunable Acrylate Resin on a Commercial DLP-SLA Printer.

We present in vivo validation of an automated magnetic hyperthermia therapy (MHT) device that uses real-time temperature input measured at the target to control tissue heating. MHT is a thermal therapy that uses heat generated by magnetic materials exposed to an alternating magnetic field. For temperature monitoring, we integrated a commercial fiber optic temperature probe containing four gallium arsenide (GaAs) temperature sensors. The controller device used temperature from the sensors as input to manage power to the magnetic field applicator. We developed a robust, multi-objective, proportional-integral-derivative (PID) algorithm to control the target thermal dose by modulating power delivered to the magnetic field applicator. The magnetic field applicator was a 20 cm diameter Maxwell-type induction coil powered by a 120 kW induction heating power supply operating at 160 kHz. Finite element (FE) simulations were performed to determine values of the PID gain factors prior to verification and validation trials. Ex vivo verification and validation were conducted in gel phantoms and sectioned bovine liver, respectively. In vivo validation of the controller was achieved in a canine research subject following infusion of magnetic nanoparticles (MNPs) into the brain. In all cases, performance matched controller design criteria, while also achieving a thermal dose measured as cumulative equivalent minutes at 43 °C (CEM43) 60 ± 5 min within 30 min.

Joseph Borrello

Cardiovascular Research Institute

Icahn School of Medicine at Mount Sinai

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Joseph Borrello

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Icahn School of Medicine at Mount Sinai

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Cardiovascular Research Institute