an-intra-tissue-radiometry-microprobe-for-measuring-radiance-situ

An Intra-Tissue Radiometry Microprobe for Measuring Radiance <em>In Situ</em> in Living Tissue

Organisms appear opaque largely because their outer tissue layers are strongly scattering to incident light; strongly absorbing pigments, such as blood, ...

Yale University

University of Pennsylvania

The opsin family of G-protein-coupled receptors are used as light detectors in animals. Opsin 5 (also known as neuropsin or OPN5) is a highly conserved opsin that is sensitive to visible violet light. In mice, OPN5 is a known photoreceptor in the retina and skin but is also expressed in the hypothalamic preoptic area (POA). Here we describe a light-sensing pathway in which POA neurons that express Opn5 regulate thermogenesis in brown adipose tissue (BAT). We show that Opn5 is expressed in glutamatergic warm-sensing POA neurons that receive synaptic input from several thermoregulatory nuclei. We further show that Opn5 POA neurons project to BAT and decrease its activity under chemogenetic stimulation. Opn5-null mice show overactive BAT, increased body temperature, and exaggerated thermogenesis when cold-challenged. Moreover, violet photostimulation during cold exposure acutely suppresses BAT temperature in wild-type mice but not in Opn5-null mice. Direct measurements of intracellular cAMP ex vivo show that Opn5 POA neurons increase cAMP when stimulated with violet light. This analysis thus identifies a violet light-sensitive deep brain photoreceptor that normally suppresses BAT thermogenesis.

Amanda L. Holt

Department of Physics

Yale University

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Amanda L. Holt

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Physics

Yale University

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Department of Physics