bilateral-renal-ischemia-reperfusion-model-for-acute-kidney-injury

Bilateral Renal Ischemia-Reperfusion Model for Acute Kidney Injury in Mice

Acute kidney injury (AKI) is defined as a rapid decline in renal function, in which persistent kidney dysfunction gradually progresses to chronic kidney ...

Zhejiang University School of Medicine

To investigate the vasodilating effect of capsaicin (CAP) on rat mesenteric artery and its mechanism.

[Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism].

Pour &eacute;tudier l&#39;effet vasodilatateur de la capsa&iuml;cine (CAP) sur l&#39;art&egrave;re m&eacute;sent&eacute;rique du rat et son m&eacute;canisme.

[L&#39;effet vasodilatateur de la capsa&iuml;cine sur art&egrave;re m&eacute;sent&eacute;rique du rat et son m&eacute;canisme].

Per indagare gli effetti vasodilatatori della capsaicina (CAP) sull&#39;arteria mesenterica del ratto e del suo meccanismo.

[Vasodilatatori effetto della capsaicina sulla arteria mesenterica del ratto e del suo meccanismo].

Chromosomal abnormalities such as aneuploidy have been shown to be responsible for causing spontaneous abortion. Genetic evaluation of abortions is currently underperformed. Screening for aneuploidy in the products of conception can help determine the etiology. We designed a high-throughput ligation-dependent probe amplification (HLPA) assay to examine aneuploidy of 24 chromosomes in miscarriage tissues and aimed to validate the performance of this technique.

A copy number variation genotyping method for aneuploidy detection in spontaneous abortion specimens.

Demethylase Tet2 plays a vital role in the immune response. Acute kidney injury (AKI) initiation and maintenance phases are marked by inflammatory responses and leukocyte recruitment in endothelial and tubular cell injury processes. However, the role of Tet2 in AKI is poorly defined. Our study determined the degree of renal tissue damage associated with Tet2 gene expression levels in a cisplatin-induced AKI mice model. Tet2-knockout (KO) mice with cisplatin treatment experienced severe tubular necrosis and dilatation, inflammation, and AKI markers' expression levels than the wild-type mice. In addition, the administration of Tet2 plasmid protected Tet2-KO mice from cisplatin-induced nephrotoxicity, but not Tet2-catalytic-dead mutant. Tet2 KO was associated with a change in metabolic pathways like retinol, arachidonic acid, linolenic acid metabolism, and PPAR signaling pathway in the cisplatin-induced mice model. Tet2 expression is also downregulated in other AKI mice models and clinical samples. Thus, our results indicate that Tet2 has a renal protective effect during AKI by regulating metabolic and inflammatory responses through the PPAR signaling pathway.

DNA demethylase Tet2 suppresses cisplatin-induced acute kidney injury.

A disintegrin and metalloproteinases (ADAMs) family are proteolytic transmembrane proteases that modulate diverse cell functions and coordinate intercellular communication. ADAMs are responsible for regulating cell proliferation, differentiation, migration, and organ morphogenesis in kidney development. Abnormally activated ADAMs drive inflammation and fibrosis in response to kidney diseases such as acute kidney injury, diabetic kidney disease, polycystic kidney disease, and chronic allograft nephropathy. ADAM10 and ADAM17, known as the most characterized members of ADAMs, are extensively investigated in kidney diseases. Notably, ADAM proteases have the potential to be targets for developing novel treatment approaches in kidney diseases.

ADAMs family in kidney physiology and pathology.

Metabolomics is useful in elucidating the progression of diabetes; however, the follow-up changes in metabolomics among health, diabetes mellitus, and diabetic kidney disease (DKD) have not been reported. This study was aimed to reveal metabolomic signatures in diabetes development and progression.

Huanhuan Zhu

Kidney Disease Center,
The First Affiliated Hospital,
Kidney Disease Center, the First Affiliated Hospital

Zhejiang University School of Medicine

Bilateral Renal Ischemia-Reperfusion Model for Acute Kidney Injury in Mice

Huanhuan Zhu

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Kidney Disease Center,The First Affiliated Hospital,Kidney Disease Center, the First Affiliated Hospital

Zhejiang University School of Medicine

Bilateral Renal Ischemia-Reperfusion Model for Acute Kidney Injury in Mice

Kidney Disease Center,
The First Affiliated Hospital,
Kidney Disease Center, the First Affiliated Hospital