analyzing-telomeric-protein-dna-interactions-using-single-molecule

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Telomeres, the protective structures at the ends of chromosomes, are crucial for maintaining cellular longevity and genome stability. Their proper ...

Nankai University

In order to probe the biological function of O-GlcNAc and the pathogenesis of associated diseases, it is essential to prepare a potent and specific O-GlcNAcase (OGA) antibody. Based on protein sequence analysis, we found N terminal 1-350 amino acids of OGA (sOGA) has high antigenicity and hydrophilicity and then constructed it into plasmid pET28a vector. First, we optimized the expression of sOGA in Escherichia coli BL21(DE3) (0.05 mmol/L IPTG, 10 hours) and purified it with the Ni-NTA affinity chromatography and size exclusion chromatography respectively. SDS-PAGE verified the molecular weight (45 kDa) and the purity (>95%) of sOGA and the purified protein was subjected to immunize New Zealand rabbits. Finally, we obtained OGA polyclonal antibody by affinity purifying the antiserum with CNBr-activated Sepharose 4B beads. Western blotting and ELISA assay showed that this antibody could recognize three OGA isoforms with high specificity and the sensitivity was 0.11 ng/mL (the titer was 1:80 000). These results indicated the prepared polyclonal antibody of OGA can be used for the biological function study of OGA.

[Antigen selection, optimized expression and polyclonal antibody preparation of O-GlcNAcase].

Wide-range, well-separated, and tunable lifetime nanocomposites with ultrabright fluorescence are highly desirable for applications in optical multiplexing such as multiplexed biological detection, data storage, and security printing. Here, a synthesis of tunable fluorescence lifetime nanocomposites is reported featuring europium chelate grafted onto the surface of plasmonic core-shell nanoparticles, and systematically investigated their optical performance. In a single red color emission channel, more than 12 distinct fluorescence lifetime populations with high fluorescence efficiency (up to 73%) are reported. The fluorescence lifetime of Eu-grafted core-shell nanoparticles exhibits a wider tunable range, possesses larger lifetime interval and is more sensitive to separation distance than that of ordinary Eu-doping core-shell type. These superior performances are attributed to the unique nanostructure of Eu-grafed type. In addition, these as-prepared nanocomposites are used for security printing to demonstrate optical multiplexing applications. The optical multiplexing experiments show an interesting pseudo-information "a rabbit in a well" and conceal the real message "NKU."

Wide-Range Tunable Fluorescence Lifetime and Ultrabright Luminescence of Eu-Grafted Plasmonic Core-Shell Nanoparticles for Multiplexing.

Rumination has been thought to relate to deficits in core executive functions (EFs), but the empirical findings for this idea are mixed. The aim of the present study is to synthesize existing literature to clarify these relations.

The relationships between rumination and core executive functions: A meta-analysis.

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Yanling Liu

State Key Laboratory of Medicinal Chemical Biology,
College of Pharmacy,
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy

Nankai University

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Yanling Liu

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}State Key Laboratory of Medicinal Chemical Biology,College of Pharmacy,State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy

Nankai University

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

State Key Laboratory of Medicinal Chemical Biology,
College of Pharmacy,
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy