micromanipulation-gene-expression-adult-zebrafish-brain-using

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Manipulation of gene expression in tissues is  required to perform functional studies. In this paper, we demonstrate the  cerebroventricular ...

Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

The zebrafish regenerates its brain after injury and hence is a useful model organism to study the mechanisms enabling regenerative neurogenesis, which is poorly manifested in mammals. Yet the signaling mechanisms initiating such a regenerative response in fish are unknown. Using cerebroventricular microinjection of immunogenic particles and immunosuppression assays, we showed that inflammation is required and sufficient for enhancing the proliferation of neural progenitors and subsequent neurogenesis by activating injury-induced molecular programs that can be observed after traumatic brain injury. We also identified cysteinyl leukotriene signaling as an essential component of inflammation in the regenerative process of the adult zebrafish brain. Thus, our results demonstrate that in zebrafish, in contrast to mammals, inflammation is a positive regulator of neuronal regeneration in the central nervous system.

Acute inflammation initiates the regenerative response in the adult zebrafish brain.

Le poisson-z&egrave;bre r&eacute;g&eacute;n&egrave;re son cerveau apr&egrave;s une blessure et est donc un organisme mod&egrave;le utile d&#39;&eacute;tudier les m&eacute;canismes permettant la neurogen&egrave;se r&eacute;g&eacute;n&eacute;ratrice, qui est mal manifeste chez les mammif&egrave;res. Pourtant, les m&eacute;canismes de signalisation initier une telle r&eacute;ponse r&eacute;g&eacute;n&eacute;ratrice chez les poissons sont inconnus. &Agrave; l&#39;aide de microinjection de c&eacute;r&eacute;broventriculaire de particules immunog&egrave;nes et dosages de l&#39;immunosuppression, nous avons montr&eacute; que l&#39;inflammation est n&eacute;cessaire et suffisante pour accro&icirc;tre la prolif&eacute;ration des cellules souches neurales et neurogen&egrave;se subs&eacute;quente en activant des programmes mol&eacute;culaires induite par le pr&eacute;judice qui peuvent &ecirc;tre observ&eacute;es apr&egrave;s traumatisme cr&acirc;nien. Nous avons &eacute;galement identifi&eacute; les leucotri&egrave;nes cyst&eacute;inyl signalisation comme une composante essentielle de l&#39;inflammation dans le processus de r&eacute;g&eacute;n&eacute;ration du cerveau adulte chez le poisson z&egrave;bre. Ainsi, nos r&eacute;sultats montrent que chez le poisson z&egrave;bre, contrairement aux mammif&egrave;res, l&#39;inflammation est un r&eacute;gulateur positif de la r&eacute;g&eacute;n&eacute;ration neuronale dans le syst&egrave;me nerveux central.

Inflammation aigu&euml; initie la r&eacute;ponse r&eacute;g&eacute;n&eacute;ratrice dans le cerveau adulte chez le poisson z&egrave;bre.

Il Danio rerio rigenera il suo cervello dopo l&#39;infortunio e quindi &egrave; un organismo modello utile per studiare i meccanismi di attivazione della neurogenesi rigenerativa, che mal si manifesta nei mammiferi. Ancora i meccanismi di segnalazione avviando una risposta rigenerativa nel pesce sono sconosciuti. Utilizzando cerebroventricular microinjection delle particelle immunogenici e dosaggi di immunosoppressione, abbiamo dimostrato che l&#39;infiammazione &egrave; necessaria e sufficiente per migliorare la proliferazione dei progenitori neurali e successiva neurogenesi attivando programmi molecolari indotta da lesioni che possono essere osservati dopo trauma cranico. Abbiamo anche individuato dei leucotrieni cisteinici segnalazione come componente essenziale dell&#39;infiammazione nel processo rigenerativo del cervello adulto zebrafish. Cos&igrave;, i nostri risultati dimostrano che in zebrafish, a differenza dei mammiferi, l&#39;infiammazione &egrave; un regolatore positivo della rigenerazione neuronale nel sistema nervoso centrale.

Anne Iltzsche

DFG-Center for Regenerative Therapies Dresden

Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Anne Iltzsche

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}DFG-Center for Regenerative Therapies Dresden

Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

DFG-Center for Regenerative Therapies Dresden