total-protein-extraction-2-d-gel-electrophoresis-methods-for

Total Protein Extraction and 2-D Gel Electrophoresis Methods for <em>Burkholderia</em> Species

The investigation of  the intracellular protein levels of bacterial species is of importance to  understanding the pathogenic mechanisms of diseases ...

University of British Columbia 

We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can convert from mucoid to nonmucoid.

Differential mucoid exopolysaccharide production by members of the Burkholderia cepacia complex.

infection with Burkholderia cepacia complex (BCC) bacteria in cystic fibrosis (CF) is associated with an unpredictable rate of pulmonary decline. Some BCC, but not others, elaborate copious mucoid exopolysaccharide, endowing them with a gross mucoid phenotype, the clinical significance of which has not been described.

Mucoid and nonmucoid Burkholderia cepacia complex bacteria in cystic fibrosis infections.

Eleven Burkholderia cepacia-like isolates of human clinical and environmental origin were examined by a polyphasic approach including recA and 16S rRNA sequence analysis, multilocus sequence analysis (MLSA), DNA base content determination, fatty acid methyl ester analysis, and biochemical characterization. The results of this study demonstrate that these isolates represent a novel species within the B. cepacia complex (Bcc) for which we propose the name Burkholderia pseudomultivorans. The type strain is strain LMG 26883(T) (=CCUG 62895(T)). B. pseudomultivorans can be differentiated from other Bcc species by recA gene sequence analysis, MLSA, and several biochemical tests including growth at 42Â°C, acidification of sucrose and adonitol, lysine decarboxylase and Î²-galactosidase activity, and esculin hydrolysis.

Trevor J. Hird

Department of Pediatrics,
Centre for Understanding and Preventing Infection in Children,
Department of Pediatrics, Centre for Understanding and Preventing Infection in Children

University of British Columbia

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Trevor J. Hird

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Pediatrics,Centre for Understanding and Preventing Infection in Children,Department of Pediatrics, Centre for Understanding and Preventing Infection in Children

University of British Columbia

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Department of Pediatrics,
Centre for Understanding and Preventing Infection in Children,
Department of Pediatrics, Centre for Understanding and Preventing Infection in Children