Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal FluidKarl T. Hansson 1, Tobias Skillbäck 1,2, Elin Pernevik 1, Jessica Holmén-Larsson 1, Gunnar Brinkmalm 1, Kaj Blennow 1,2, Henrik Zetterberg 1,2,3, Johan Gobom 1,2
1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3Department of Molecular Neuroscience, UCL Institute of Neurology
A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.
