Atomic Force Microscopy Measurements of Cartilage in Intact and Regenerating Axolotl LimbsSandra Edwards-Jorquera *1,2, Rita Aires *1,2, Elke Ulbricht 3, Anna Taubenberger 3, Tatiana Sandoval-Guzmán 1,2,4
1Department of Internal Medicine III, Center for Healthy Aging, University Hospital Carl Gustav Carus, Technische Universität Dresden (TUD), 2Center for Regenerative Therapies Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden (TUD), 3Biotechnology Center, Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden (TUD), 4Paul Langerhans Institute Dresden, Helmholtz Centre Munich, University Hospital Carl Gustav Carus, Technische Universität Dresden (TUD)
In this protocol, we show how to prepare axolotl tissue for atomic force microscopy (AFM) and perform indentation measurements in intact and regenerating limb cartilage.
