Universitat de Barcelona

4 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
Xavier Morató *1,2, Marc López-Cano *1,2, Paula M. Canas 3, Rodrigo A. Cunha 3, Francisco Ciruela 1,2
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra

Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.

image

Neuroscience

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
Vanessa Gil 1,2,3,4, José Antonio Del Río 1,2,3,4
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona

Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

image

Neuroscience

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis
Marina Cunquero 1, Esther Aguilar 2, Pablo Loza-Alvarez 1, Jesús Planagumà 1,2
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Universitat de Barcelona

Autoimmune encephalitis is a new category of antibody-mediated diseases of the central nervous system. Hippocampal neurons can be used to discover and characterize these antibodies. This article provides a protocol for primary cell culture and immunostaining to determine autoantibodies in the serum and cerebrospinal fluid of patients.

image

Medicine

Real-Time Cardiac Mapping with a Noninvasive Imageless Electrocardiographic Imaging System
Jana Reventos-Presmanes 1,2,3, Carlos Fambuena 2,3, Andrea Cano-Cabañero 2, Maria Correas 2, Ismael Hernández-Romero 2,3, Clara Herrero-Martin 2, David Lundback 2,3, Ines Llorente 2, Marta Martinez 2, Raúl Moreno-Lopez 2, Rubén Molero 2,3, Javier Milagro 3, Berta Pellicer-Sendra 1,3, Mariona Regany 1, Santiago Ros 2,4, Jaume Serrano 1,3, Jorge Vicente 3, Ernesto Zacur 3, Till Althoff 1, Elena Arbelo 1,5,6, Angel Arenal 4, Pablo Ávila 4, Alejandro Carta-Bergaz 4, Eduard Guasch 1,5,6, Jean-Baptiste Guichard 1, Maite Izquierdo 7, Margarida Pujol-López 1,5, Andreu Porta-Sánchez 1,5,6, Ivo Roca-Luque 1,5,6, José M. Tolosana 1,5,6, Esteban G. Torrecilla 4, Joaquin Osca 7, Lluís Mont 1,5,6, Felipe Atienza 3,4, Maria S. Guillem 2,3, Andreu M. Climent 2,3
1Arrhythmia Section, Institut Clínic Cardiovascular, Hospital Clínic, Universitat de Barcelona, 2ITACA Institute, Universitat Politècnica de València, 3Corify Care SL, 4Arrhythmia Section, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), 5Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 6Centro de Investigación Biomédica En Red de enfermedades CardioVasculares (CIBERCV), 7Arrhythmia Section, Hospital Universitari i Politècnic La Fe

This study introduces a novel approach for real-time cardiac mapping using a noninvasive, imageless electrocardiographic imaging system. This system enables the acquisition of electrophysiological cardiac maps without requiring pre-procedural computed tomography or magnetic resonance imaging scans, enabling efficient guidance for cardiac procedures such as ablation and cardiac resynchronization therapy implants.