Geisel School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Jacob Buford¹, Owen Wilkins², Fred Kolling IV², Steven D. Leach², Duncan Morhardt^1,3

¹Department of Surgery, Dartmouth Hitchcock Medical Center, ²Dartmouth Cancer Center, ³Dartmouth College, Geisel School of Medicine

Here, we present a method for aligning and cryosectioning multiple Zebrafish (Danio rerio) larvae samples and collecting them on a single slide for spatial transcriptomic analysis.