Sungkyunkwan University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Intraspinal Cavity Injection of Human Mesenchymal Stem Cells and Tracking their Migration into the Rat Brain

Hyeongseop Kim^1,2, Seunghoon Lee³, Jong Wook Chang^1,2, A ran Kim⁴, Hyemin Jang^5,6,7, Duk L. Na^5,6,7

¹Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, ²Stem Cell Institute, ENCell Co. Ltd, ³Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, ⁴Animal Research and Molecular Imaging Center, Samsung Biomedical Research Institute, Samsung Medical Center, ⁵Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, ⁶Neuroscience Center, Samsung Medical Center, ⁷Samsung Alzheimer Research Center, Samsung Medical Center

Several routes of administration can be used to deliver mesenchymal stem cells (MSCs) to the brain. In the present study, MSCs were delivered throughout the neuraxis and brain via intra-spinal cavity injection. MSCs were injected into the spinal cavities of rats, and stem cell migration was tracked and quantified.

Biochemistry

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Joohyun Lee¹, Truc Kim¹, Kyeong Kyu Kim¹

¹Department of Precision Medicine, Graduate School of Basic Medical Science (GSBMS), Institute for Antimicrobial Resistance Research and Therapeutics, Sungkyunkwan University School of Medicine

This protocol presents a practical method using Methanocaldococcus jannaschii (MjsHSP16.5) as an example to address uneven particle distribution through sample preparation optimization, providing a reference for researchers to efficiently elucidate macromolecular structures using cryogenic electron microscopy (cryo-EM).