The Geisel School of Medicine at Dartmouth

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
Leah M. Rommereim 1, Miryam A. Hortua Triana 1, Alejandra Falla 1, Kiah L. Sanders 1, Rebekah B. Guevara 1, David J. Bzik 1, Barbara A. Fox 1
1Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth

Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.

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