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US Army Medical Research Institute of Chemical Defense

1 ARTICLES PUBLISHED IN JoVE

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Biology

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury
John G. Lehman 1, Robert D. Causey 1, Cristina V. LaGrasta 1, Albert L. Ruff 1
1US Army Medical Research Institute of Chemical Defense

High throughput small inhibitory RNA screening is an important tool that could help to more rapidly elucidate the molecular mechanisms of chemical cornea epithelial injury. Herein, we present the development and validation of exposure models and methods for the high throughput screening of hydrogen fluoride- and chloropicrin-induced cornea epithelial injury.

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