Aalborg University Hospital

6 ARTICLES PUBLISHED IN JoVE

Medicine

Basic Surgical Techniques in the Göttingen Minipig: Intubation, Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion

Kaare S. Ettrup^1,2, Andreas N. Glud², Dariusz Orlowski², Lise M. Fitting¹, Kaare Meier¹, Jens Christian Soerensen¹, Carsten R. Bjarkam^1,2, Aage K. Olsen Alstrup³

¹Department of Neurosurgery, Aarhus University Hospital, ²Department of Neurobiology, Institute of Anatomy, Faculty of Health Sciences, Aarhus University, ³Positron Emission Tomography (PET) Centre, Aarhus University Hospital

The use of domestic and miniature pigs in science has increased significantly in recent years. By demonstrating how to perform intubation, transurethral bladder catheterization, femoral artery and vein catheterization, as well as transcardial perfusion, we aim to further increase the value of Göttingen minipigs in biomedical research.

JoVE Core

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Carsten R. Bjarkam¹, Dariusz Orlowski², Laura Tvilling², Johannes Bech², Andreas N. Glud², Jens-Christian H. Sørensen²

¹Department of Neurosurgery, Clinical Institute of Medicine, Aalborg University Hospital, ²Center of Experimental Neuroscience (Cense), Department of Neurosurgery, Institute of Clinical Medicine, Aarhus University Hospital

The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.

Medicine

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

Noud van Helmond¹, Hans Timmerman¹, Søren S. Olesen², Asbjørn M. Drewes², Joris Kleinhans³, Oliver H. Wilder-Smith¹, Kris C. Vissers¹, Monique A. Steegers¹

¹Department of Anesthesiology, Pain, and Palliative Medicine, Radboud University Medical Center, ²Mech-Sense, Department of Gastroenterology & Hepatology, Aalborg University Hospital, ³Medical Technology & Clinical Physics, Radboud University Medical Center

Persistent postsurgical pain may be related to changes in pain processing. By assessing pain in response to standardized stimuli, changes in pain processing can be elucidated. We present methods to obtain pain thresholds to different stimuli and a measure of endogenous analgesia in patients undergoing breast cancer surgery.

JoVE Core

Testing of all Six Semicircular Canals with Video Head Impulse Test Systems

Dan D. Hougaard^1,2, Emil R. Abrahamsen^1,2

¹Department of Otolaryngology, Head & Neck Surgery and Audiology, Aalborg University Hospital, ²Department of Clinical Medicine, Aalborg University

This protocol describes how to correctly perform the video head impulse test with two separate test systems commonly used worldwide. Both the 2D and 3D video head impulse test methods are described.

Neuroscience

Accessing the Porcine Brain via High-Speed Pneumatic Drill Craniectomy

Theis Mariager^1,2, Jacob Holmen Terkelsen¹, Carsten Reidies Bjarkam¹

¹Department of Neurosurgery, Aalborg University Hospital, ²Department of Infectious Diseases, Aalborg University Hospital

This protocol describes performing a craniectomy using a high-speed pneumatic drill on a 3-month-old Danish Landrace pig. The access is made through the frontal bone and reveals the ventral dura mater and underlying cerebral hemispheres. This procedure allows for access to a large portion of the pig brain.

Neuroscience

Surgical Technique for Lumbar Spinal Catheter Insertion in Pigs Enabling Continuous Access to the Thecal Sac in a Terminal Setup

Theis Mariager^1,2, Jacob Holmen Terkelsen¹, Pernille Veiss-Pedersen³, Carsten Reidies Bjarkam¹

¹Department of Neurosurgery, Aalborg University Hospital, ²Department of Infectious Diseases, Aalborg University Hospital, ³Department of Radiology, Aalborg University Hospital

We present a technique for inserting a lumbar spinal catheter at the L4-L5 level in a 3-month-old Danish Landrace pig as part of a terminal research protocol, enabling continuous infusion or CSF sampling from the thecal sac.