Åbo Akademi University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Mahlet Z. Tamirat¹, Kari J. Kurppa², Klaus Elenius^2,3,4, Mark S. Johnson¹

¹Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, ²Medicity Research Laboratories and Institute of Biomedicine, University of Turku, ³Turku Bioscience Centre, University of Turku and Åbo Akademi University, ⁴Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital

The objective of this protocol is to use molecular dynamics simulations to examine the dynamic structural changes that occur due to activating mutations of the EGFR kinase protein.

Biology

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Janine Grolleman^1,2, Iris M. T. Pijnenburg^1,2, Carlijn V. C. Bouten^1,2, Vito Conte^1,2,3, Cecilia M. Sahlgren^1,2,4

¹Department of Biomedical Engineering, Soft Tissue Engineering and Mechanobiology, Eindhoven University of Technology, ²Institute for Complex Molecular Systems, Eindhoven University of Technology, ³Institute for Bioengineering of Catalonia, The Barcelona Institute of Science and Technology, ⁴Faculty of Science and Engineering, Cell Biology, Åbo Akademi University

Sprouting angiogenesis, fundamental for development and disease, involves complex molecular and mechanical processes. We present a versatile 2.5D ex vivo model that analyzes cellular sprouting from porcine carotid arteries, revealing stiffness-dependent angiogenesis and distinct leader-follower cell mechanics. This model aids in advancing tissue engineering strategies and cancer therapy approaches.