Universidade Nova de Lisboa (ITQB NOVA)

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Gonçalo M. Poças^1,2, Pedro M. Domingos^*2, Christen K. Mirth^*1

¹School of Biological Sciences, Monash University, ²Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)

Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.

Biology

Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles

Jorge M. S. Faria^1,2, A. Cristina Figueiredo^3,4, Dora M. Teixeira⁵, Maria L. Inácio^1,2

¹INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., ²GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), ³Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁴Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁵Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School, University of Évora

The protocol describes the in vivo and in vitro pinewood nematode infection of Pinus pinaster and their volatilome analysis through Gas Chromatography (GC) and GC coupled to Mass Spectrometry (GC-MS).

Biology

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

Jorge M. S. Faria^1,2, Pedro Barbosa^3,4, A. Cristina Figueiredo⁴, Manuel Mota³, Cláudia S. L. Vicente³

¹INIAV, I.P., National Institute for Agrarian and Veterinary Research, ²GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), ³MED – Mediterranean Institute for Agriculture, Environment and Development & CHANGE – Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, ⁴Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa

The protocol describes infection of Solanum tuberosum roots with plant parasitic nematodes under in vivo greenhouse conditions and potato in vitro transgenic roots for histochemical analysis of root structure through optical microscopy.