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Pasteur Institute of Tunis

1 ARTICLES PUBLISHED IN JoVE

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Biology

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Elhem Yacoub 1, Imen Chniba 1, Nadine Khadraoui 1, Béhija Mlik 1, Boutheina Ben Abdelmoumen Mardassi 1
1Group of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Pasteur Institute of Tunis

In Mycoplasma pneumoniae infection, serology tests can generate good results, yet with low specificity because of immunological cross-reaction. The in-house antigen-capture ELISA, described in this paper, guarantees high species specificity and has been shown to be a reliable screening test for accurate diagnosis of M. pneumoniae.

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