Università di Milano-Bicocca

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules

Luisa Fiandra¹, Serena Mazzucchelli¹, Marta Truffi¹, Michela Bellini², Luca Sorrentino¹, Fabio Corsi¹

¹Dipartimento di Scienze Biomediche e Cliniche Luigi Sacco, Università di Milano, ²Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca

A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.

Cancer Research

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Marta Truffi^*1, Leopoldo Sitia^*2, Marta Sevieri², Arianna Bonizzi², Maria Antonietta Rizzuto³, Serena Mazzucchelli², Fabio Corsi^1,2

¹Istituti Clinici Scientifici Maugeri IRCCS, ²Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università di Milano, ³Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment.

Bioengineering

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Alessandra Nardini^1,2,3, Behjat Sadat Kariman⁴, Mario Marini⁵, Conci Conci¹, Marco Grassi¹, Margaux Bouzin⁵, Maddalena Collini⁵, Roberto Osellame², Giulio Cerullo⁴, Manuela Teresa Raimondi¹, Giuseppe Chirico⁵, Rebeca Martínez Vázquez²

¹Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, ²Istituto di Fotonica e Nanotecnologie (IFN), Consiglio Nazionale delle Ricerche (CNR), ³Department of Experimental Medicine, Università del Salento, ⁴Department of Physics, Politecnico di Milano, ⁵Department of Physics, Università di Milano-Bicocca

This protocol describes the fabrication of an implantable integrated imaging window using 3D laser printing. The window consists of a system of microlenses coupled with micro-scaffolds. The method involves two-photon polymerization (2PP) of the biocompatible photoresist SZ2080 in a continuous sequence, optimizing manufacturing efficiency and alignment between the different components.