Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression

Podsumowanie

Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression.

Streszczenie

Chronic spinal cord compression is the most common cause of spinal cord impairment in patients with nontraumatic spinal cord damage. Conventional magnetic resonance imaging (MRI) plays an important role in both confirming the diagnosis and evaluating the degree of compression. However, the anatomical detail provided by conventional MRI is not sufficient to accurately estimate neuronal damage and/or assess the possibility of neuronal recovery in chronic spinal cord compression patients. In contrast, diffusion tensor imaging (DTI) can provide quantitative results according to the detection of water molecule diffusion in tissues. In the present study, we develop a methodological framework to illustrate the application of DTI in chronic spinal cord compression disease. DTI fractional anisotropy (FA), apparent diffusion coefficients (ADCs), and eigenvector values are useful for visualizing microstructural pathological changes in the spinal cord. Decreased FA and increases in ADCs and eigenvector values were observed in chronic spinal cord compression patients compared to healthy controls. DTI could help surgeons understand spinal cord injury severity and provide important information regarding prognosis and neural functional recovery. In conclusion, this protocol provides a sensitive, detailed, and noninvasive tool to evaluate spinal cord compression.

Wprowadzenie

Chronic spinal cord compression is the most common cause of spinal cord impairment1. This condition can be due to posterior longitudinal ligament ossification, hematoma, cervical disc herniation, vertebral degeneration, or intraspinal tumors^2,3. Chronic spinal cord compression can lead to various degrees of functional deficits; however, there are clinical cases with serious spinal cord compression without any neurological symptoms and signs, as well as patients with mild spinal cord compression but serious neurological deficits⁴. Under these circumstances, sensitive imaging is essential to evaluate compression severity and identify the range of damage.

Conventional MRI plays a significant role in elucidating spinal cord anatomy. This technique is usually utilized to evaluate the compression degree because of its sensitivity to soft tissues⁵. Many parameters can be measured from MRI, such as MR signal intensity, cord morphology, and spinal canal area. However, MRI has some limitations and only provides qualitative information rather than quantitative results⁶. Patients with chronic spinal cord compression often have abnormal signal changes of MRI intensity. However, discrepancies between clinical symptoms and MRI intensity changes make it hard to diagnose a functional condition based solely on MRI characteristics⁷. Previous studies highlight this controversy in terms of the prognostic value of MRI T2 hyperintensity in the spinal cord8. Two groups reported that T2 hyperintensity of the spinal cord is a poor prognostic parameter after surgery for chronic spinal cord compression8,9. In contrast, some authors found no significant association between T2 signal changes and prognosis^8,9. Chen et al. and Vedantam et al. divided MRI T2 hyperintensities into two categories corresponding to different prognostic outcomes^10,11. Type 1 showed faint, fuzzy, indistinct borders, and this category demonstrated reversible histologic changes. Type 2 images presented intense, well-defined borders, which corresponded to irreversible pathologic damage. Conventional T1/T2 MRI techniques do not provide adequate information to identify these two categories and evaluate patient prognosis. By contrast, DTI, a more sophisticated imaging technique, may help obtain more specific prognostic information by quantitatively detecting microstructural changes in tissues via water molecule diffusion.

In recent years, DTI has garnered increasing attention due to its ability to describe spinal cord microarchitecture. DTI can measure the direction and magnitude of water molecule diffusion in tissues. DTI parameters can quantitatively evaluate neural damage in patients with chronic spinal cord compression. FA and the ADC are the most commonly applied parameters during spinal cord evaluation. The FA value reveals the degree of anisotropy to orientate surrounding axonal fibers and describe anatomic boundaries^12,13. The ADC value provides information on the characteristics of molecular motion in many directions in a three-dimensional space and reveals the mean of diffusivities along the three principal axes^6,12. Changes in these parameters are associated with microstructural alterations that influence water molecule diffusion. Therefore, surgeons can utilize/measure DTI parameters to identify spinal cord pathology. The present study provides DTI methods and processes that provide more detailed prognostic information to treat patients with chronic spinal cord compression.

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Protokół

The study was approved by the local Medical Ethics Committee in Guangzhou First People’s Hospital in China. Signed informed consent forms were received from healthy volunteers and participants prior to participation. All of the studies were conducted in accordance with the World Medical Association Declaration of Helsinki.

1. Subject Preparation

1. Ensure that each participant meets the following criteria for chronic spinal cord compression: a) a history of loss of significant neurological function, b) a positive myelopathy physical examination, and c) MRI evidence of cervical cord compression. 
   NOTE: The exclusion criteria are a) incapability of providing written consent and b) inability to obtain DTI parameters of artifacts. For controls, inclusion criteria are a) no history of significant back or neck injuries, neurological disorders, or spine surgeries; b) no MRI evidence of cervical cord compression.
2. sk each participant to complete and sign a consent form that lists MRI safety guidelines and the imaging protocol. Specifically, patients with chronic spinal cord compression are examined by MRI preoperatively and 1 year postoperatively.
3. rovide earplugs for each participant. Place them in a supine position with a head/neck coil enclosing the cervical region, and a landmark at the thyroid cartilage level. Ensure that each participant is in a comfortable position that effectively reduces movement.

2. Structural MRI Parameters 

NOTE: Anatomical T1-weighted (T1 W) images, T2-weighted (T2 W) images, and DTI acquired on a 3 Tesla MRI scanner with a 16-channel head coil.

1. Use fast perturbation gradient echo (FPGR) for localization scanning to obtain axial, sagittal, and coronal position maps.
2. Position the sagittal positioning line with the coronal position maps to ensure that the positioning baseline is parallel to the spinal canal (spinal cord); first locate the sagittal plane T2 W, then copy and paste the sagittal T1 W positioning line to the T2 W positioning line.
   1. Use the following imaging parameters for T1 W and T2 W sagittal imaging: field of view (FOV) = 240 mm x 240 mm, voxel size = 1.0 mm x 0.8 mm x 3.0 mm, slice gap = 0.3 mm, slice thickness = 3 mm, number of excitation (NEX) = 2, fold-over direction = feet/head (FH), and time of echo (TE)/time of repetition (TR) = 10/700 ms (T1 W) and 101/2,500 ms (T2 W). Obtain nine sagittal images covering the entire cervical spinal cord. 
3. Position the axial positioning line on the sagittal T2 W image and cover the intervertebral disc from C2/3 to C6/7, centering on the anteroposterior diameter of the intervertebral space. Use the following imaging parameters: FOV = 180 mm x 180 mm, voxel size = 0.7 mm x 0.6 mm x 3.0 mm, slice thickness = 3 mm, fold-over direction = anterior/posterior (AP), NEX = 2, and TE/TR = 120/3,000 ms.
4. Position the axial positioning line on the sagittal T2 W image, centering on the anteroposterior diameter of the intervertebral space, with 45 slices covering the cervical spinal cord from C1 to C7.
   1. Obtain DTI via the following sequence: single-shot spin-echo echo-planar imaging (SE-EPI) with 20 orthogonal directions. Non-coplanar diffusion directions with b-value = 800 s/mm². 
   2. Use the following imaging parameters: FOV = 230 mm x 230 mm, acquisition matrix = 98 x 98, reconstructed resolution = 1.17 x 1.17, slice thickness = 3 mm, fold-over direction = AP, NEX = 2, EPI factor = 98, and TE/TR = 74/8,300 ms. Provide a time course summarizing the steps in the MRI protocol, as shown in Figure 1. 
      NOTE: The time course summarizing the MRI and DTI protocol is shown in Figure 1.

3. Image Postprocessing and Data Measurement Indexes

1. Automatically convey all scanning images to the Syngo MR B17. Load the T2 W sagittal and axial imaging of the intervertebral space in the filming interface and find the most compressed portion of the cervical spinal cord.
2. In the 2:1 viewing interface, load the FA image and click the Position Display: Series tab. Count and record the level of highest compression from the top to the bottom of the location map.
3. Click the File tab to select the tensor image, then use the applications toolbar at the top left of the screen to select Neuro 3D(MR) to automatically create ADC and FA colormaps.
4. Turn to the level of the highest compression site, and create spherical regions of interest (ROIs) of identical volumes (with a size of 6 mm³) using the Start Evaluation Mode tab. The ROIs must be selected, including the inner spinal cord to exclude the partial volume effects of cerebrospinal fluid (CSF).
5. Calculate and display the FA and ADC values at the bottom right of the screen automatically. Display the E1, E2, and E3 values by clicking the Diffusion toolbar and choosing them.
   NOTE: All measurements were performed by two radiologists blinded to the patients’ clinical details. The final results were determined as the average of the two.
6. Perform image processing of the DTI datasets using a Syngo MR B17 Advantage Workstation, following the steps in Figure 2.

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Wyniki

This is a summary of results obtained from healthy volunteers and patients with cervical spondylotic myelopathy. The protocol enabled the physician to view DTI maps. This technology could serve as an objective measure to measure functional status in myelopathic conditions. DTI maps of healthy volunteers are shown in Figure 3. The DTI parameters of healthy volunteers were as follows: FA = 0.661; ADC = 1.006 x 10^-3 mm²/s; E1 = 1.893 x 10^-3 mm²/s; E2 ...

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Dyskusje

Conventional MRI is usually utilized to assess the prognosis of patients with various spine conditions. However, this imaging modality provides macroscopic anatomic detail rather than microstructure evaluation¹⁴, which limits the prediction of neurological function. Furthermore, traditional MRI may underestimate the severity and extent of spinal cord damage. The emergence of DTI can help surgeons to evaluate spinal cord function more accurately by providing quantitative information on water molecu...

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Materiały

Name	Company	Catalog Number	Comments
3-Tesla MRI scanner	Siemens	40708	Software: NUMARIS/4
Syngo MR B17	Siemens	40708	Software: NUMARIS/4