Begin by selecting 10 six-week old male mice and placing them into two groups labeled WT and CKO, with five mice in each group. Administer Tamoxifen to all the mice every two days for a week. Prepare a plastic mouse dissection platform as an operating table to immobilize the mice.
Attach an elastic band to each rubber post for limb fixation. Tie another thread to the metal rod to hold the upper incisors. Place the anesthetized mouse in the supine position on the operating table.
Use four elastic bands to fix the limbs. One thread attached to the metal rod to hold the upper incisors. Next, prepare closed coil springs for orthodontic force application.
Ligate one end of the prepared spring to the maxillary left first molar. Ensure a stable force of 10 grams by measuring with the dynamometer. Then ligate the other end to the central incisor using a 0.1 millimeter resin reinforced steel ligature wire.
After the operation, place the mice in a recovery cage. Monitor the mice for two to four hours until they regain sufficient consciousness to maintain sternal recumbency. Provide a soft diet and observe the mice regularly for complications, administering analgesic drugs as needed.