Begin the immunization of the mouse by performing a PT intravenous injection into its lateral caudal vein. To do so, gently plug the tail of the properly anesthetized mouse with an ethanol solution, which acts as a vasodilator to make the veins visible. Focusing on one of the two lateral veins of the tail, use 0.5 milliliter syringe fitted with a 30 gauge needle to inject 500 nanograms of PT.Next, proceed to administer subcutaneous injections of MOG 35 to 55 emulsion previously prepared in one milliliter syringes.
Using the syringes with 26 gauge needles, perform two subcutaneous injections of 100 microliters each under the rostral part of the flanks. Then administer one injection of 100 microliters at the base of the tail.
