Neonatal Mouse Bone Marrow Isolation and Preparation of Bone Marrow-Derived Macrophages

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1.3K Views

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06:53 min

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May 24th, 2024

DOI :

10.3791/66613-v

May 24th, 2024

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Madhavi Annamanedi¹, Jordan K. Vance¹, Cory M. Robinson¹^,²

¹Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, ²Vaccine Development Center, West Virginia University Health Sciences Center

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Neonatal Mice

Bone Marrow Isolation

Macrophage Preparation

Bone Marrow derived Macrophages

Differentiation

Phagocytic Efficiency

Flow Cytometry

Tissue Remodeling

L929 conditioned Medium

Surface Markers

Rozdziały w tym wideo

0:00

Introduction

1:10

Non‐Enzymatic Method for Isolating Neonatal Mouse Bone Marrow Cells

2:42

Differentiation of Neonatal Bone Marrow‐Derived Macrophages Using a Supernatant of L929 Cells

4:37

Assessing Phagocytic Function of Neonatal Bone Marrow‐Derived Macrophages Using a Fluorescent Bacterial Uptake Assay

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