DNA Extraction from Cancer Tissues for the Detection of Low-Frequency Mutations Using Blocker Displacement Amplification Technology

To begin, prepare all the reagents required for tissue sample preparation and DNA extraction. Add 500 microliters of deep paraffin buffer, and 20 microliters of proteinase K solution to about 30 milligrams of tissue in a micro centrifuge tube. Vortex the micro centrifuge tube for at least 10 seconds and place it in a dry thermostat set at 55 degrees Celsius for 90 minutes.

After incubation, transfer the sample including liquid and tissue to the spin column that is placed in a filter tube. Centrifuge at 16, 500 G for five minutes to separate the liquid into the filter tube. Then, transfer the liquid from the filter tube to a labeled sample tube, and perform DNA extraction according to the manufacturer's instructions.

Measure the concentration and purity of the extracted DNA.