To begin, remove the prepared agarose tube with added beads from a 37 degree Celsius water bath. Pour the entire 10 milliliters of agarose onto a six centimeter Petri dish. Once the temperature drops below 33 degrees Celsius, transfer 10 to 15 embryos into the agarose solution.
Swirl the Petri dish so that the little buffer that would've been transferred gets well dispersed. Next, take a 200 microliter micropipette with an appropriate pipette tip and insert a clean straight tube taken out of the microcentrifuge tube into the pipette tip. Using the micropipette, aspirate a little agarose into the tube, followed by two to three embryos.
Without releasing the pressure from the pipette, detach the tube from the pipette tip and place it on the lid of the Petri dish filled with E3 so that the agarose can solidify. Once the agarose has solidified, transfer the tubes to a two milliliter microcentrifuge tube filled with E3.
