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Refined Multiview Light Sheet Microscopy Protocol for Zebrafish Embryos

July 19th 2024


* These authors contributed equally


Summary

A sample preparation strategy for imaging early zebrafish embryos within an intact chorion using a light-sheet microscope is described. It analyzes the different orientations that embryos acquire within the chorion at the 70% epiboly and bud stages and details imaging strategies for obtaining cellular-scale resolution throughout the embryo using the light-sheet system.

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