Begin by assembling the sample holder to locate the embryo. Insert the tube with embryos directly into a capillary of the right diameter. Now start live scanning and navigate using the specimen navigator to select the first view.
Set up the slice interval, followed by the first and last slices of the Z-stack in this view. Change the angle in the specimen navigator to move to the next view. Set up the Z-stack for the second view and add the information in the multi-view dialog box.
After setting all the views, save this information in a text file that contains the Z-stack information, coordinates of the tube, and angle specifications for each view. Then clear all positions from the multi-view dialog box. Next, translate to a different Y-coordinate away from the embryo where beads are visible.
Add this position to the multi-view dialog box and save this position in a new text file. Then go to the folder where the text files are saved and duplicate the Embryo Positions file to a new file named Beads Positions. Replace the Y-coordinate for all the views in the Beads Positions file with the Y-coordinate from the Beads Y file.
Afterwards, return to the imaging software and load the Beads Positions file. If the time series option is activated, select one cycle and start the experiment. Save bead images as beads"to register different views during image processing.
Then clear the positions and load the initial Embryo Positions file in the software, set the desirable number of cycles with a suitable time interval and start the experiment.
