JoVE Journal

Medicine

This content is Open Access.

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using ³¹P NMR Spectroscopy

Please note that all translations are automatically generated. Click here for the English version.

26.1K Views

•

11:38 min

•

August 31st, 2010

DOI :

August 31st, 2010

•

Stephen C. Kolwicz Jr.¹, Rong Tian¹

¹Department of Anesthesiology & Pain Medicine, University of Washington School of Medicine

Podsumowanie

Langendorff-mode isolated heart perfusion, in conjunction with ³¹P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.

Rozdziały w tym wideo

0:00

Title

0:59

Introduction

1:25

Experimental Set-up

4:33

Isolated Heart Preparation

9:43

Representative Results of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

7:41

Assessment of Cardiac Function and Energetics Using 31P NMR Spectroscopy

11:09

Conclusion

Powiązane Filmy

article

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

17.9K Views

article

Reduction in Left Ventricular Wall Stress and Improvement in Function in Failing Hearts using Algisyl-LVR

24.2K Views

article

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats

12.7K Views

article

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

27.5K Views

article

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

22.7K Views

article

Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy

7.4K Views

article

Assessment of Kidney Function in Mouse Models of Glomerular Disease

17.4K Views

article

Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism

13.0K Views

article

Echocardiographic Assessment of Cardiac Anatomy and Function in Adult Rats

20.5K Views

article

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

5.9K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej