Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety

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15.1K Views

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14:03 min

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July 24th, 2011

DOI :

10.3791/2822-v

July 24th, 2011

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Bonnie Barrilleaux¹, Paul Knoepfler¹

¹Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:18

Production of Lentivirus

4:03

Titering the Lentivirus

4:57

Transduction of Human Target Cells with Murine Retrovirus Receptor Slc7a1

5:50

Polymer Complex Titration to Determine Toxicity

10:15

Ecotropic Transduction with Polymer Complexation

11:08

Results of the Procedure

13:32

Conclusion

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Lentivirus Production

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