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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

14.6K Views

12:13 min

October 8th, 2012

DOI :

10.3791/3761-v

October 8th, 2012


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TransFLP

Rozdziały w tym wideo

0:05

Title

1:16

Preparation of Chitin Flakes

1:39

Oligonucleotide Design and Polymerase Chain Reaction

3:45

Chitin-induced Natural Transformation

4:59

Removal of Selective Cassette(s) by FLP-recombination

5:46

Plasmid Curing

11:34

Conclusion

6:21

Results: Representative TransFLP Modifications of V. cholerae

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