We thank Dr. Randy Chitwood for critically reading the manuscript. This work was supported by the Whitehall Foundation and the Alfred P. Sloan Foundation grants to HJK.

1. Optical Setup (Figure 1)
<ol>
 <li>For two-photon excitation an infrared pulsed laser system with femtosecond pulses is used. A high laser output power (in some cases &gt;2W at 890 nm wavelength) is required to offset the large losses introduced by the optical components of the system.</li>
 <li>A prechirper system consisting of two prisms imparts a negative group velocity dispersion (GVD) onto the laser pulses prior to the acousto-optical deflectors (AODs) to compensate for the temporal dispersion introduced by the AODs1.</li>
 <li>Two AODs with large apertures (10 mm for a 40x water immersion objective with NA 0.8) deflect the laser beam in two dimensions.</li>
 <li>A reflective diffraction grating with 100 groves/mm is placed 13 cm behind the AODs to compensate the spatial dispersion introduced by AODs when using short laser pulses.</li>
 <li>The laser beam is directed with two relay telescopes into the camera port of an upright microscope.</li>
 <li>Irises are placed at regular intervals for alignment of the optical components.</li>
 <li>A dichroic beamsplitter in front of the objective transmits the infrared excitation light to the specimen and reflects the fluorescence light from the specimen onto a detector.</li>
 <li>Epi- and transfluorescence detectors (photomultipliers, PMTs) collect fluorescence signal through the objective and - if applicable - through the condenser.</li>
 <li>Colored glass filters (BG-39, 3-5 mm) are placed in front of the detectors to prevent excitation light reaching the detectors.</li>
 <li>The AOD deflection angles are controlled by a computer equipped with a digital-analog-converter board (156.25 kHz clock rate), which in turn drives voltage-controlled oscillators.</li>
 <li>The signal from the photomultipliers is relayed through a low-pass Butterworth filter (cut-off frequency of 100 kHz) and digitized by an analog-digital-converter (156.25 kHz clock rate) before being stored in a computer for analysis.</li>
 <li>Alignment and electrical noise are tested by recording the distribution of fluorescence signals with and without laser light, at low and high gain of the photomultipliers, as well as with and without indicator. The scanner is correctly setup and shielded when the width of the distribution of the fluorescence signals at high gain and with indicator is much larger than the widths of the other distributions of fluorescence signals (Figure 2).</li>
</ol>
2. Experimental Procedures
<ol>
 <li>Dithered random-access scanning relies on detection of intracellular increases in calcium. A large number of neurons can be stained using bolus injection of the ester form of a calcium indicator (for example Oregon Green 488 Bapta-1 AM) into neural tissue5.</li>
 <li>Several locations from each neuron soma are recorded, each for a short time ("dithering", 4 locations, 6.4 &mu;sec for each location = 25.6 &mu;sec recording time for each neuron in each cycle, Figure 3C). To select neurons of interest a full frame consisting of 256x256 pixels is acquired (Figure 3A). The center of each neuron soma to be recorded is selected manually within this image. The control software automatically adds three points at 2 &mu;m distance around this center.</li>
 <li>In each cycle, fluorescence signal is recorded from each of the 40 neurons (Figure 3B). This process is repeated for the entire duration of one recording (5 sec recording = 3,255 cycles, 1 cycle = 1.536 msec).</li>
</ol>
3. Online Software Ttools to Maximize Spike Detection Efficiency
<ol>
 <li>Spike detection from fluorescence somatic calcium signals relies on a high signal-to-noise (S/N) ratio of the somatic fluorescence calcium signals. A high S/N can be achieved by increasing excitation intensity. Excitation intensity, however, can only be increased to a certain limit because of photodamage. Spike detection is high within a very small window of excitation intensities only where fluorescence signals have a high S/N but only very little photodamage is observed3. In order to ensure that the recorded signals are within the window of high spike detection during recordings we monitor photon rate (see equation 3.2) and decline of baseline fluorescence using online analysis.</li>
 <li>Approximate photon rate per neuron is calculated from a short time window (100-200 ms) of baseline noise. Number of photons (N&lambda;) and photon rate (&lambda; = N&lambda; / &Delta;t) is calculated from the fluorescence values by fitting the distribution (&sigma;) of relative fluorescence changes with the following equation:</li>
</ol>
<img alt="Equation 1" src="/files/ftp_upload/4052/4052eq1.jpg" /> 
This equation represents the poisson distribution for photon shot noise with a change of variable to relative fluorescence change: &Delta;F/F = (G*N&lambda;(t)-G*N&lambda;,0)/G*N&lambda;,0 where G denotes the cumulative gain of photomultiplier and all other electronic components. Note that this equation does not correctly determine the number of detected photons for in vivo recordings because there are other sources of noise (movement artifacts), in addition to photon shot noise. Nevertheless this equation is useful for in vivo recordings to estimate the noise.
<ol start="3">
 <li>Baseline fluorescence is calculated from the same time window and plotted as a function of time or trials. The average decline of baseline is kept below 0.0002/sec by adjusting laser power because spike detection rapidly declines when exceeding this limit.</li>
 <li>Every 10-20 min the neuron somata positions are verified by again acquiring a full frame image. If required, recording locations are adjusted. Locations can be adjusted for all neurons at once, or for individual neurons.</li>
</ol>
4. Reconstruction of Spike Timings from Fluorescence Signals (Deconvolution)
<ol>
 <li>The fluorescence signals resulting from neural activity often summate in time because the decay of the calcium transients is long (several hundred millisec). A deconvolution method reconstructs spike and spike timings from fluorescence signals.</li>
 <li>To determine the most likely spike train underlying the recorded fluorescence signal, different models are compared. Here we used a genetic algorithm to determine the model - and thus the spike train and spike timings - with the maximum likelihood.</li>
 <li>In inhomogeneous populations of neurons, the spike-evoked calcium signal can vary between neurons. For unsupervised analysis of data sets we designed an algorithm that takes into account the variation of the spike-evoked calcium signal from neuron to neuron.</li>
 <li>To avoid a large number of false positive detections it is useful to constrict the allowed amplitude and decay time constant of the model of the spike-evoked calcium signal. The joint distribution of the amplitude and decay time constant of single-spike evoked calcium transients are recorded in a separate set of experiments from the same type of neurons under the same experimental conditions using simultaneous electrophysiological and optical recordings.</li>
 <li>To account for slow baseline changes and to reduce computational costs of deconvolving, longer recordings are divided into several shorter traces of 1-5 sec.</li>
 <li>For each neuron and each recording, the deconvolution algorithm may test a large number of models (up to 1,000,000 different models or more). To speed up deconvolution, one experiment is deconvolved on up to 10 different computers in parallel.</li>
 <li>After deconvolution, the spike data is analyzed and inspected. A peri-stimulus time histogram, spike probability, and firing rate (average spike per neurons) are calculated in an automated manner.</li>
</ol>
5. Representative Results
Successful spike detection hinges on a high signal-to-noise ratio of the recorded fluorescence somatic calcium signals. Simply using high excitation rates (high laser power) can result in an adverse impact of photoeffects on biological material (photodamage). In dithered random-access scanning photodamage manifests as decreases in baseline fluorescence and decreases of the spike-evoked calcium fluorescence signals. The decrease in the spike-evoked signal can quickly result in a failure to detect spikes. There is only a very small window of excitation intensity where spike detection from fluorescence signals is high. On the higher end this window is limited by photodamage, on the lower end the fluorescence signals have a low signal-to-noise ratio. For cortical neurons in acute slices we use laser power resulting in photon rates of about 400,000-1,500,000 photon/sec when recording at around 100 &mu;m below slice surface. When using a high-affinity indicator - here Oregon Green 488 BAPTA - 1 - this signal is sufficient to detect individual spikes. Figure 3E shows an example of a fluorescence signal recorded at very low excitation rate, one example of a recording within the detection window, and one at very high excitation rate.
Compared to other techniques to record neural activity with single-cell and single-spike resolution, dithered random-access scanning can record from a larger number of neurons from the same, local population, and is less invasive for example compared to tetrode/multielectrode recordings. Thus dithered random-access scanning can be used to record neural activity from many neurons to measure mutual information signaled by suprathreshold activity6 (Figure 4A), changes of neural activity in a population of neurons (cortical plasticity), and propagation of suprathreshold activity through populations of neurons14 (Figure 4B)
<img alt="Figure 1" src="/files/ftp_upload/4052/4052fig1.jpg" /> 
Figure 1. Optical design of the dithered-random access scanning setup.
<img alt="Figure 2" src="/files/ftp_upload/4052/4052fig2.jpg" /> 
Figure 2. Alignment and testing: distributions of fluorescence signals recorded under different conditions. A) No laser light and low photomultiplier gain, B) At higher PMT gain, but no laser light, the distribution is wider because of the photomultiplier dark current. C) With the laser on and recorded at high PMT gain. A difference between the distributions in shown in B and this distribution would indicate that excitation light reaches the PMT detectors. D) The distribution of fluorescence signals recorded at high gain from neuron somata. If no other noise source contributes, this distribution arises from photon shot-noise only.
<img alt="Figure 3" src="/files/ftp_upload/4052/4052fig3.jpg" /> 
Figure 3. A) Full frame fluorescence image to detect and select neuron somata positions, B) Scan path of one cycle, C) Illustration of the dithering principle; in each soma (circle) several locations are recorded before moving the beam to the next soma, D) Illustration of the output of the two D/A channels. For each neuron soma, fluorescence signal is recorded from 4 different spots in each soma (s1-s4). The location of each spot is given by its x and y position. The x and y positions for all spots and all neurons are sent to the digital-to-analog converter in a sequential manner. While the beam is moved between two neuron somata, no signal is acquired (blank). E) Examples of fluorescence signals. Note that each example shows response to one spike (as measured with electrophysiological cell-attached recording).
<img alt="Figure 4" src="/files/ftp_upload/4052/4052fig4.jpg" /> 
Figure 4. Studying cortical function using dithered random-access scanning. A) Measuring mutual information signaled by populations of neurons. Upper image shows photomicrograph of an acute brain slice and two stimulation pipettes placed in the same cortical column in layer 4(L4). Center graphs show neural responses for each repetition of a stimulus. Lower graph shows Shannon's mutual information signaled by the recorded population of neurons. B) Measuring propagation of suprathreshold spiking activity (signal propagation) between populations of cortical neurons. Upper graph illustrates experimental design, center image shows fluorescence image, dashed lines indicate barrel borders, lower graph shows detected spikes in response to electrical stimulation of thalamocortical fibers (triangles).

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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+recording+of+neuronal+spiking+activity+from+unbiased+populations+of+neurons+with+high+spike+detection+efficiency+and+high+temporal+precision.">Optical recording of neuronal spiking activity from unbiased populations of neurons with high spike detection efficiency and high temporal precision.</a> J. Neurophysiol. 104, 1812-1824 (2010).</li><li>Grewe, B. F., Langer, D., Kasper, H., Kampa, B. M., Helmchen, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-speed+in+vivo+calcium+imaging+reveals+neuronal+network+activity+with+near-millisecond+precision.">High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision.</a> Nat. 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No conflicts of interest declared.

Dithered random-access scanning indirectly detects suprathreshold spiking activity from the increases in intracellular somatic calcium associated with each spike in a neuron somata. The increases in intracellular calcium are detected by fluorescent calcium dyes. The limitations of dithered random-access scanning arise largely from of the limited signal-to-noise ratio of the calcium fluorescence signals. The signal-to-noise ratio is in turn limited by photodamage, which does not allow using high excitation rates. Because ...

<table border="1">
	<tbody>
		<tr>
			<td>Name of the reagent</td>
			<td>Company</td>
			<td>Catalogue number</td>
			<td>Comments (optional)</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Optical components are listed in order, starting from the laser</td>
		</tr>
		<tr>
			<td>Titan:Sapphire Laser</td>
			<td>Coherent Inc.</td>
			<td>Chameleon Ultra 2</td>
			<td>High power output recommended (&gt;2W at 900 nm)</td>
		</tr>
		<tr>
			<td>Achromatic lens f = 30 mm</td>
			<td>Thor labs</td>
			<td>AC254-030-B</td>
			<td>Anti-reflection (AR) coating for 650-1050 nm</td>
		</tr>
		<tr>
			<td>Achromatic lens f = 100 mm</td>
			<td>Thor labs</td>
			<td>AC254-100-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>lens f = 75 mm</td>
			<td>Thor labs</td>
			<td>LA1608-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>lens f = 175 mm</td>
			<td>Thor labs</td>
			<td>LA1229-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>Achromatic lens f = 300 mm</td>
			<td>Thor labs</td>
			<td>AC254-300-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>Achromatic lens f = 100 mm</td>
			<td>Thor labs</td>
			<td>AC254-100-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>Achromatic lens f = 100 mm</td>
			<td>Thor labs</td>
			<td>AC254-100-B</td>
			<td>AR 650-1050 nm</td>
		</tr>
		<tr>
			<td>Acousto-optical deflectors</td>
			<td>Intraaction Corp</td>
			<td>ATD 6510CD2</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Reflective diffraction grating</td>
			<td>Newport</td>
			<td>53-011R</td>
			<td>100 grooves/mm for AODs with 65 MHz bandwidth and scan angle of 45 mrad</td>
		</tr>
		<tr>
			<td>21.6 mm Brewster prisms</td>
			<td>Lambda Research Optics Inc.</td>
			<td>IBP21.6SF10</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Colored Glass</td>
			<td>Schott</td>
			<td>BG-39</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dichroic mirror</td>
			<td>Chroma Technology Corp</td>
			<td>Z532RDC</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Photomultiplier modules</td>
			<td>Hamamatsu</td>
			<td>H9305-03</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>DAC-ADC board</td>
			<td>National Instruments</td>
			<td>PCI-6115</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Oregon Green 488 Bapta-1 AM</td>
			<td>Invitrogen</td>
			<td>O-6807</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>

optical recording of suprathreshold neural activity with single-cell and single-spike resolution

Signaling of information in the vertebrate central nervous system is often carried by populations of neurons rather than individual neurons. Also propagation of suprathreshold spiking activity involves populations of neurons. Empirical studies addressing cortical function directly thus require recordings from populations of neurons with high resolution. Here we describe an optical method and a deconvolution algorithm to record neural activity from up to 100 neurons with single-cell and single-spike resolution. This method relies on detection of the transient increases in intracellular somatic calcium concentration associated with suprathreshold electrical spikes (action potentials) in cortical neurons. High temporal resolution of the optical recordings is achieved by a fast random-access scanning technique using acousto-optical deflectors (AODs)1. Two-photon excitation of the calcium-sensitive dye results in high spatial resolution in opaque brain tissue2. Reconstruction of spikes from the fluorescence calcium recordings is achieved by a maximum-likelihood method. Simultaneous electrophysiological and optical recordings indicate that our method reliably detects spikes (&gt;97% spike detection efficiency), has a low rate of false positive spike detection (&lt; 0.003 spikes/sec), and a high temporal precision (about 3 msec) 3. This optical method of spike detection can be used to record neural activity in vitro and in anesthetized animals in vivo3,4.

Watch this Scientific Journal Video about Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution at JoVE.com

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Understanding the function of the vertebrate central nervous system requires recordings from many neurons because cortical function arises on the level of populations of neurons. Here we describe an optical method to record suprathreshold neural activity with single-cell and single-spike resolution, dithered random-access scanning. This method records somatic fluorescence calcium signals from up to 100 neurons with high temporal resolution. A maximum-likelihood algorithm deconvolves the underlying suprathreshold neural activity from the somatic fluorescence calcium signals. This method reliably detects spikes with high detection efficiency and a low rate of false positives and can be used to study neural populations in vitro and in vivo.

Signaling of information in the vertebrate central nervous system is often carried by populations of neurons rather than individual neurons. Also ...

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11.8K Views.  The University of Texas at Austin. Understanding the function of the vertebrate central nervous system requires recordings from many neurons because cortical function arises on the level of populations of neurons. Here we describe an optical method to record suprathreshold neural activity with single-cell and single-spike resolution, dithered random-access scanning. This method records somatic fluorescence calcium signals from up to 100 neurons with high temporal resolution. A maximum-likelihood algorithm ...