JoVE Journal

Biology

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Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

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111.2K Views

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09:01 min

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September 15th, 2012

DOI :

September 15th, 2012

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Ashleigh C. McLean¹^,²^,³, Nicolas Valenzuela³^,⁴, Stephen Fai³^,⁴, Steffany A.L. Bennett¹^,³

¹Department of Biochemistry, Microbiology and Immunology, Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, ²Department of Cellular and Molecular Medicine, University of Ottawa , ³CIHR Program in Neurodegenerative Lipidomics, University of Ottawa , ⁴Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism

Podsumowanie

Here, we describe how to identify the stage of the murine reproductive (proestrus, estrus, metestrus, or diestrus) by simple, non-invasive collection and cytological assessment of vaginal smear samples. We further describe how vaginal cytology reflects circulating hormonal levels underlying transition through the murine reproductive cycle.

Rozdziały w tym wideo

0:00

Title

2:48

Introduction

3:26

Preparing Reagents

3:46

Obtaining the Vaginal Smear

5:06

Cytological Staining using Crystal Violet

5:46

Vaginal Cytology

7:13

Conclusion

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