JoVE Science Education

Biology I: yeast, Drosophila and C. elegans

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English

Yeast Transformation and Cloning

Przegląd

S. cerevisiae are unicellular eukaryotes that are a commonly-used model organism in biological research. In the course of their work, yeast researchers rely upon the fundamental technique of transformation (the uptake of foreign DNA by the cell) to control gene expression, induce genetic deletions, express recombinant proteins, and label subcellular structures.

This video provides an overview of how and why yeast transformation is carried out in the lab. The important features of yeast plasmids will be presented, along with the procedure required to prepare yeast cells to incorporate new plasmids. The presentation also includes a step-by-step protocol for the lithium acetate method of yeast transformation. Finally, examples of the many applications of this essential technique will be provided.

Procedura

Yeast or Saccharomyces cerevisiae, is a widespread simple eukaryotic model organism used in the study of genetics and cell biology that can give insights into human cellular processes. This video discusses transformation - the uptake of foreign DNA by the yeast cell. It will introduce yeast plasmids, how to prepare yeast cells for transformation, a step-by-step transformation procedure, and will provide some applications of this fundamental technique.

Before we talk about the tra

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0:00

Overview

0:41

Important Aspects of Yeast Transformation

2:42

Principles Behind Yeast Transformation

3:38

Lithium Acetate Transformation Procedure

6:31

Applications

8:04

Summary

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