This work was supported by the Marie Curie International Reintegration Grant and by the German-Israel Foundation. We acknowledge Mr. Yair Razvag for AFM analysis.

1. Self-assembly of Homoaromatic Dipeptides
<ol>
	<li>Weigh the desired peptide in its lyophilized form (e.g. NH2-Phe-Phe-OH, Boc-Phe-Phe-COOH) and prepare a stock solution by dissolving the peptide in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) to the appropriate concentration (e.g. 100 mg/ml for NH2-Phe-Phe-OH and Boc-Phe-Phe-COOH)7,17,46.</li>
	<li>Mix the solution&#160;using vortex and place on the bench until the peptide&#160;is&#160;completely dissolved and the solution&#160;seems clear (a few minutes).</li>
	<li>Dilute the peptide stock solution, with a suitable solvent, to the appropriate concentration (e.g. 2 mg/ml of NH2-Phe-Phe-OH in triple distilled water (TDW) for the formation of nanotubes; by adding 2 &#956;l of the peptide stock solution to 98 &#956;l TDW, 5 mg/ml of Boc-Phe-Phe-COOH in ethanol for the formation of spherical structures).</li>
	<li>Keep the solution at RT for 24 hr.</li>
	<li>In order to avoid any preaggregation, prepare fresh stock solutions for each experiment.</li>
</ol>
2. Coassembly of Two Homoaromatic Dipeptides
<ol>
	<li>Prepare a solution of 50% ethanol by mixing equal volumes of TDW and absolute ethanol. Use vortex to mix the two solutions.</li>
	<li>Weigh 2 mg of the NH2-Phe-Phe-OH peptide and 1 mg of the Boc-Phe-Phe-OH peptide. Dissolve each peptide in HFP to a concentration of 100 mg/ml.</li>
	<li>Mix the peptides stock solutions using vortex and place them on the bench until the peptides are completely dissolved and the solutions seem clear.</li>
	<li>Blend the peptides stock solutions to the desired ratio. In this specific experiment blend 10 &#956;l of the NH2-Phe-Phe-OH peptide with 6 &#956;l of the Boc-Phe-Phe-OH peptide (to a final ratio of 5:3 respectively). Due to the high volatility of the HFP solvent, it is recommended to prepare a large amount of this stock solution (at least 10 &#956;l).</li>
	<li>Use vortex to mix the blended peptides stock solution.</li>
	<li>Dilute the blended peptides stock solution with 50% ethanol to the desired final concentration. In this specific experiment, in order to obtain a final concentration of 5 mg/ml for NH2-Phe-Phe-OH and 3 mg/ml for Boc-Phe-Phe-OH respectively, add 8 &#956;l of the blended peptides stock solution to 92 &#956;l of the 50% ethanol solution. Use a pipette to gently mix the solution.</li>
	<li>Keep the solution at RT for 24 hr.</li>
	<li>It should be noted that due to the highly volatile nature of the solvent, the experiments are sensitive to small changes in the concentration of the peptides. Therefore, fresh stock solutions should be prepared for each experiment.</li>
</ol>
3. Characterization of the Self Assembled Structures Using Scanning Electron Microscopy (SEM)
<ol>
	<li>After 24 hr of incubation, apply a 10 &#956;l drop of the peptides solution on a glass cover slip and dry at RT.</li>
	<li>Coat the sample on the glass with a thin layer of gold (a few nanometers) using a sputter coater for 90&#160;sec.</li>
	<li>Image the assemblies using SEM operating at 10-20 kV.</li>
</ol>
4. Characterization of the Self Assembled Structures Using Transmission Electron Microscopy (TEM)
<ol>
	<li>Place a 10 &#956;l drop of the peptides solution on a 200-mesh copper grid covered with carbon and stabilized by a polymer film support.</li>
	<li>After 1 min remove the excess fluid using filter paper.</li>
	<li>Prepare a solution of 2% uranyl acetate in TDW. Filter the solution using 0.22 &#956;m filter unit.</li>
	<li>To stain the sample (negative staining), place a drop of 10 &#956;l uranyl acetate solution on the grid.</li>
	<li>After 30 sec remove excess fluid using filter paper. It should be noted that although negative staining improves the contrast of the images, it is not essential in all cases.</li>
	<li>Image the sample on the grid by TEM operating at 120 kV.</li>
</ol>
5. Three-dimensional Characterization of the Assemblies by Atomic Force Microscopy (AFM)
<ol>
	<li>Prepare a sample for the AFM analysis using the procedure described in paragraph 3.1.</li>
	<li>Analyze the sample on the glass using an AFM instrument working in AC mode. Use silicon cantilevers with a spring constant of 3 N/m and a resonant frequency of 75 kHz.</li>
	<li>Start by scanning a large area of the grid, in order to find the desired structure. Then focus on a specific smaller area and scan it (the scan size was 2.5 &#956;m&#160;x&#160;2.5 &#956;m for the image included in this manuscript).</li>
</ol>
6. Characterization of the Secondary Structure by FT-IR
<ol>
	<li>Apply a 30 &#956;l drop of the peptides solution to a CaF2 window.</li>
	<li>Allow the solution to dry at RT.</li>
	<li>The adsorption of water in the IR spectrum is at 1,650 cm-1. This peak is at the center of the amide I band of the peptide bond. It is also a typical peak for &#945;-helical structures of peptides and proteins47. In order to overcome this problem and avoid the signal of water, a hydrogen-to-deuterium exchange must be performed. Place a drop of deuterium oxide (D2O) on the dried peptide sample. The drop should be large enough to completely cover the peptide deposit on the window.</li>
	<li>Allow the sample to dry under vacuum.</li>
	<li>Repeat steps 6.3 and 6.4 2x to ensure maximal hydrogen-to-deuterium exchange. Save the sample under vacuum until its analysis.</li>
	<li>Record the FT-IR spectra using a deuterated triglycine sulfate (DTGS) detector. The FT-IR system includes a purge gas generator, in order to prevent humidity in the surroundings of the sample. For samples of short peptides, it is best to scan the sample 2,000x at a resolution of 4 cm-1. The transmittance minimal values can be determined by the software supplied with the instrument.</li>
</ol>

The authors declare that they have no competing financial interests.

In summary, this paper demonstrates the ease in which peptide-based assemblies can be formed in vitro. The process involves commercially available peptides and solvents, and it occurs spontaneously under ambient conditions, upon the addition of a polar solvent to the test tube. It is crucial to use HFP as a solvent of the peptides, due to the low solubility of the peptides in other organic solvents. In addition, due to the high volatility of HFP it is necessary to prepare fresh stock solution for each experiment...

Nature forms ordered and functional structures by the process of biomolecular self-assembly. Understanding the forces that govern this spontaneous process may lead to the ability to mimic self-assembly in vitro and consequently to major advances in the area of material sciences10,11. Peptides, specifically, hold great promise as a biomolecular building block, since they present large structural diversity, ease of chemical synthesis, and can easily be functionalized with biological and chemical entities. The field of peptide self-assembly was pioneered by Ghadiri and his colleagues, who demonstrated the self-assembly of peptide nanotubes by cyclic peptides with alternating D- and L-amino acids12. Other successful approaches to the design of peptide assemblies include linear bolaamphiphile peptides5,&#160;amphiphiles (AP)6, nonconjugated self-complementary ionic peptides13, surfactant-like peptides4,14, and diblock copolypeptides15.
A more recent approach involves the self-assembly of short aromatic peptides, termed homoaromatic dipeptides. These peptides comprise only two amino acids with aromatic nature (e.g. Phe-Phe, tert-butyl dicarbonate (Boc)-Phe-Phe)7,8,16-21. The structures formed by these homoaromatic peptides include tubular structures, spheres, sheet-like assemblies and fibers6,8,15,21-32. The fibers in some cases generate a fibril mesh that yields a hydrogel33-37. These assemblies have been exploited for applications of biosensing, drug delivery, molecular electronics, etc.38-45
This paper describes the experimental steps needed in order to start the spontaneous self-assembly of homoaromatic peptides. In addition, it presents the process of peptide coassembly. This process involves the self-assembly of more than one type of peptide monomer.
Our demonstration includes the coassembly of two commercially available peptides: the diphenylalanine peptide (NH2-Phe-Phe-COOH) and its Boc protected analogue (Boc-Phe-Phe-OH). Each of the peptides self-assembles into a supermolecular structure: the diphenylalanine peptide forms tubular assemblies and the Boc-Phe-Phe-OH peptide self-assembles into either spheres or fibers depending on the solvent7,17,46. We blended the two peptides in certain ratios and characterized the resulted assemblies by electron microscopy, force microscopy and FT-IR spectroscopy. The methods demonstrated the formation of a peptide-based structure which is comprised of spherical elements with a diameter of several microns (1-4 &#956;m) that are connected by elongated assemblies with a diameter of a few hundred nanometers (~300-800 nm). The assemblies resemble beaded strings in their morphology,&#160;as the spherical structures seem to be threaded on the elongated assemblies. We therefore termed these assemblies &#34;biomolecular necklaces&#34;. The &#34;biomolecular necklaces&#34; might serve as a new biomaterial, as a drug delivery agent or as a scaffold for electronic applications. Moreover, the procedure that leads to the self-assembly of peptides may be utilized with other classes of peptides and biomolecules. It may lead to a better understanding of the forces involved in self-assembly and the formation of new ordered structures.

<table border="1">
	<tbody>
		<tr>
			<td>Name of Material/ Equipment</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments/Description</td>
		</tr>
		<tr>
			<td>NH2-Phe-Phe-OH</td>
			<td>Bachem</td>
			<td>G-2925.0001</td>
			<td></td>
		</tr>
		<tr>
			<td>Boc-Phe-Phe-OH</td>
			<td>Bachem</td>
			<td>A-3205.0005</td>
			<td></td>
		</tr>
		<tr>
			<td>1,1,1,3,3,3-hexafluoro-2-propanol</td>
			<td>Sigma-Aldrich</td>
			<td>52512-100ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol absolute (Dehydrated) AR sterile</td>
			<td>Bio-Lab Ltd.</td>
			<td>52555</td>
			<td>Blending with TDW for the preparation of 50% solution</td>
		</tr>
		<tr>
			<td>Uranyl acetate</td>
			<td>Sigma-Aldrich</td>
			<td>73943</td>
			<td>For negative staining. It is possible to work without it.</td>
		</tr>
		<tr>
			<td>glass cover slip</td>
			<td>Marienfeld Laboratory Glassware</td>
			<td>110590</td>
			<td></td>
		</tr>
		<tr>
			<td>TEM grids</td>
			<td>Electron Microscopy Sciences</td>
			<td>FCF200-Cu-50</td>
			<td>Formvar/Carbon 200 Mesh, Cu</td>
		</tr>
		<tr>
			<td>Quantitive filter paper</td>
			<td>Whatman</td>
			<td>1001055</td>
			<td></td>
		</tr>
		<tr>
			<td>Deuterium Oxide (D2O)</td>
			<td>Sigma-Aldrich</td>
			<td>151882-100G</td>
			<td>99.9 atom % D</td>
		</tr>
		<tr>
			<td>CaF2 window</td>
			<td>PIKE Technologies</td>
			<td>160-1212</td>
			<td>25 mm x 2 mm window. For FT-IR measurments</td>
		</tr>
		<tr>
			<td>AFM tips</td>
			<td>NanoScience Instruments</td>
			<td>CFMR</td>
			<td>Aspire probes, CFMR-25 series</td>
		</tr>
		<tr>
			<td>Filter units</td>
			<td>Millipore</td>
			<td>SLGV033RS</td>
			<td>Millex-GV, 0.22 &#956;m, PVDF, 33 mm, gamma sterilized</td>
		</tr>
		<tr>
			<td>SEM</td>
			<td>FEI</td>
			<td></td>
			<td>Quanta 200 ESEM</td>
		</tr>
		<tr>
			<td>TEM</td>
			<td>FEI</td>
			<td></td>
			<td>Tecnai T12 G2 Spirit</td>
		</tr>
		<tr>
			<td>AFM</td>
			<td>JPK Instruments</td>
			<td></td>
			<td>A JPK NanoWizard3</td>
		</tr>
		<tr>
			<td>FT-IR</td>
			<td>Thermo Fisher Scientific</td>
			<td></td>
			<td>Nicolet 6700 advanced gold spectrometer</td>
		</tr>
		<tr>
			<td>FT-IR Purge</td>
			<td>Parker</td>
			<td></td>
			<td>BALSTON FT-IR Purge Gas Generator model 75-52</td>
		</tr>
		<tr>
			<td>OMNIC (Nicolet) software</td>
			<td>Thermo Nicolet Corporation</td>
			<td></td>
			<td>For FT-IR spectra analysis</td>
		</tr>
		<tr>
			<td>Vortex mixer</td>
			<td>Wisd Laboratory Equipment</td>
			<td></td>
			<td>ViseMix VM</td>
		</tr>
		<tr>
			<td>Weight</td>
			<td>Mettler Toledo</td>
			<td></td>
			<td>NewClassic MS</td>
		</tr>
		<tr>
			<td>Sputter coater</td>
			<td>Polaron</td>
			<td></td>
			<td>SC7640 Sputter Coater</td>
		</tr>
	</tbody>
</table>

formation of ordered biomolecular structures by the self-assembly of short peptides

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control self-assembly and mimicking this process in vitro will bring about major advances in the areas of materials science and nanotechnology. Among the available biological building blocks, peptides have several advantages as they present substantial diversity, their synthesis in large scale is straightforward, and they can easily be modified with biological and chemical entities1,2. Several classes of designed peptides such as cyclic peptides, amphiphile peptides and peptide-conjugates self-assemble into ordered structures in solution. Homoaromatic dipeptides, are a class of short self-assembled peptides that contain all the molecular information needed to form ordered structures such as nanotubes, spheres and fibrils3-8. A large variety of these peptides is commercially available.
This paper presents a procedure that leads to the formation of ordered structures by the self-assembly of homoaromatic peptides. The protocol requires only commercial reagents and basic laboratory equipment. In addition, the paper describes some of the methods available for the characterization of peptide-based assemblies. These methods include electron and atomic force microscopy and Fourier-Transform Infrared Spectroscopy (FT-IR). Moreover, the manuscript demonstrates the blending of peptides (coassembly) and the formation of a &#34;beads on a string&#34;-like structure by this process.9 The protocols presented here can be adapted to other classes of peptides or biological building blocks and can potentially lead to the discovery of new peptide-based structures and to better control of their assembly.

This paper describes a method for the formation of ordered structures at the nano-and micrometer scale by the self-assembly of peptides. In order to demonstrate this simple process we present and characterize the coassembly of two simple aromatic peptides (Figure 1). One of the peptides is the NH2-Phe-Phe-OH (diphenylalanine) peptide, which can self-assemble in an aqueous solution into hollow tubular structures with nanometric dimensions7. The other peptide is its Boc protected anal...

Watch this Scientific Journal Video about Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides at JoVE.com

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

This paper describes the formation of highly ordered peptide-based structures by the spontaneous process of self-assembly. The method utilizes commercially available peptides and common lab equipment. This technique can be applied to a large variety of peptides and may lead to the discovery of new peptide-based assemblies.

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control ...

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12.7K Views.  The Hebrew University of Jerusalem. This paper describes the formation of highly ordered peptide-based structures by the spontaneous process of self-assembly. The method utilizes commercially available peptides and common lab equipment. This technique can be applied to a large variety of peptides and may lead to the discovery of new peptide-based assemblies.