Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
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11.4K Views
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08:30 min
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April 30th, 2014
DOI :
April 30th, 2014
•Ashim Gupta1, Kevin Sharif2, Megan Walters2, Mia D. Woods1, Anish Potty2, Benjamin J. Main4, Saadiq F. El-Amin III1,2,3
1Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, 4University of Illinois at Springfield
Rozdziały w tym wideo
0:05
Title
1:23
Tissue Digestion and hACL Isolation
2:16
Cellular Expansion, Freezing and Thawing
3:26
Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
4:22
Scanning Electron Microscopy (SEM)
5:54
Immunofluorescence Staining
7:01
Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
8:02
Conclusion
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