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Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

11.4K Views

08:30 min

April 30th, 2014

DOI :

10.3791/51597-v

April 30th, 2014


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Keywords Anterior Cruciate Ligament

Rozdziały w tym wideo

0:05

Title

1:23

Tissue Digestion and hACL Isolation

2:16

Cellular Expansion, Freezing and Thawing

3:26

Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold

4:22

Scanning Electron Microscopy (SEM)

5:54

Immunofluorescence Staining

7:01

Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface

8:02

Conclusion

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