Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Please note that all translations are automatically generated. Click here for the English version.

9.7K Views

•

08:33 min

•

December 4th, 2014

DOI :

10.3791/51853-v

December 4th, 2014

•

Soumya Narayan¹, Charlee McLean², Akira Sawa¹, Sandra Y. Lin³, Narayan Rai², MariaMananita S. Hipolito², Nicola Cascella⁴, John J.I. Nurnberger, Jr.⁵, Koko Ishizuka¹, Evaristus A. Nwulia²

¹Department of Psychiatry, Johns Hopkins University, ²Department of Psychiatry and Behavioral Sciences, Howard University, ³Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University, ⁴Department of Psychiatry, Sheppard Pratt Hospital, ⁵Department of Psychiatry, Indiana University

Podsumowanie

Przeglądaj więcej filmów

Keywords Olfactory Neurons

Laser capture Microdissection

Bipolar Disorder

Lithium Carbonate

Neuropsychiatric Disorders

Olfactory Epithelium

Treatment Response

Rozdziały w tym wideo

0:05

Title

1:49

Laser-Capture Microdissection

3:48

Isolation of Total RNA and cDNA Synthesis

5:50

Real Time PCR Quantification

7:14

Reults: GSK-3β Expression in OE Neurons

7:53

Conclusion

Powiązane Filmy

article

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

16.7K Views

article

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

30.6K Views

article

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

15.4K Views

article

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

5.2K Views

article

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

21.2K Views

article

A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation

8.9K Views

article

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

23.9K Views

article

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

26.7K Views

article

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

8.5K Views

article

Investigation of Activated Mouse Olfactory Sensory Neurons via Combined Immunostaining and in situ Hybridization

1.2K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone